L-Iditol Production from L-Sorbose by a Methanol Yeast, Candida boidinii (Kloeckera sp.) No. 2201
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概要
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Production of L-iditol (iditol) from L-sorbose (sorbose) with D-sorbitol dehydrogenase coupled with NADH regeneration under methanol oxidation was studied with the resting cell system of a methanol yeast, Candida boidinii (Kloeckera sp.) no. 2201.Maximum activities of iditol production and enzymes concerned with the production were found in cells grown on a medium containing methanol and D-xylose (xylose). The highest amount of iditol, 142-148 g/l (94-98% conversion rate), was obtained from 150 g/l of sorbose in the presence of 0.5 M methanol at pH 6.5. Intermittent control of the pH during the cell reaction gave a significantly higher amount of iditol than that obtained without such control. Freeze-thawing cells showed rather higher productivity than resting cells. The product was identified as iditol without contamination of its C-2 epimer, D-sorbitol, by high performance liquid chromatography.
- 1988-10-25
著者
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Vongsuvanlert Vitchuporn
Research Center For Cell And Tissue Culture Faculty Of Agriculture
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Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
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