Production of L-Methionine-Enriched Cells of a Mutant Derived from a Methylotrophic Yeast, Candida boidinii
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概要
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L-Methionine-enriched cells production of an ethionine-resistant mutant of Candida boidinii no. 2201 was greatly improved by the control of pH and by feeding of methanol and other medium components during cultivation in a jar fermentor. Under the optimal conditions, 38.5 g (as dry weight) of cells and 282 mg of pool methionine (intracellular pool of free-L-methionine) per l of culture broth were obtained after 11 d of cultivation.The culture conditions for production of L-methionine-enriched cells in continuous culture were investigated. With limited methanol in continuous cultivation, pool methionine productivity reached a maximum value of 1.14 mg・l^<-1>・h^<-1> at a dilution rete of 0.05・h^<-1>. During methanol-limited growth in continuous cultivation, the pool methionine content of the mutant was about 20-35% higher than that in batch cultivation.
- 公益社団法人日本生物工学会の論文
- 1988-12-25
著者
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Lim W‐j
Kyoto Univ. Kyoto Jpn
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Lim Wang-jin
Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University
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Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
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