Enzymatic Synthesis of D-Cysteine by 3-Chloro-D-alanine Resistant Pseudomonas putida CR 1-1
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概要
- 論文の詳細を見る
3-Chloro-D-alanine chloride-lyase, which occurs in the cells of Pseudomonas putida CR 1-1, catalyzes not only the α, β-elimination reaction of 3-chloro-D-alanine to form pyruvate, but also its β-replacement reaction in the presence of a high concentration of sodium hydrosulfide to form D-cysteine. Using the β-replacement reaction, the enzymatic synthesis of D-cysteine by resting cells was investigated. The culture conditions for cell production of the bacterium with high D-cysteine-producing activity and the reaction conditions for D-cysteine production were optimized. Under these optimal reaction conditions, 100% of the added 3-chloro-D-alanine could be converted to D-cysteine and, as the highest yield, 20.6mg of D-cysteine per 1.0ml of reaction mixture could be synthesized.
- 社団法人 日本農芸化学会の論文
著者
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Nagasawa Toru
Department Of Agricultural Chemistry Kyoto University
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HOSONO Hidekazu
Department of Agricultural Chemistry, Kyoto University
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YAMANO Hironori
Department of Agricultural Chemistry, Kyoto University
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OHKISHI Haruyuki
Central Research Laboratory, Mitsubishi Chemical Industries
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Tani Yoshiki
Research Center For Cell And Tissue Cluture Faculty Of Agriculture Kyoto University
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Yamada Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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OHKISHI Haruyuki
Central Research Laboratories, Mitsubishi Chemical Industries
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