Bioconversion of 1-adamantanol to 1,3-adamantanediol using Streptomyces sp. SA8 oxidation system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
To efficiently produce 1,3-adamantanediol (1,3-ad(OH)_2) from 1-adamantanol (1-adOH), our stocks of culture strains and soil microorganisms were surveyed for hydroxylation activity towards 1-adOH. Among them, the soil actinomycete SA8 showing the highest hydroxylation activity was identified as Streptomyces sp. based on 16S ribosomal DNA sequence analysis. The reaction products were purified by silica gel column chromatography, and from NMR and MS analyses, they were identified as 1,3-ad(OH)_2 and 1,4-ad(OH)_2. Streptomyces sp. SA8 produced 5.9gl^<-1> 1,3-ad(OH)_2 from 6.2gl^<-1> 1-adOH in culture broth after 120h at 25℃. Using resting cells, 2.3gl^<-1> 1,3-ad(OH)_2 was produced after 96h of incubation at a 69% conversion rate. In both cases, 1,4-ad(OH)_2 was formed as a byproduct at a rate of about 15%. Strain SA8 also hydroxylated 2-adamantanol and 2-methyl-2-adamantanol.
著者
-
Mitsukura Koichi
Department of Biomolecular Science, Gifu University
-
Sakamoto Hiromu
Department of Biomolecular Science, Gifu University
-
Kubo Haruka
Department of Biomolecular Science, Gifu University
-
Yoshida Toyokazu
Department of Biomolecular Science, Gifu University
-
Nagasawa Toru
Department of Biomolecular Science, Gifu University
-
Kubo Haruka
Department Of Biomolecular Science Gifu University
-
Nagasawa Toru
Department Of Agricultural Chemistry Kyoto University
-
Sakamoto Hiromu
Department Of Biomolecular Science Gifu University
-
Mitsukura Koichi
Department Of Biomolecular Science Gifu University
-
Yoshida Toyokazu
Department Of Biomolecular Science Gifu University
-
Nagasawa Toru
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Yoshida Toyokazu
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Mitsukura Koichi
Department Of Biomolecular Science Faculty Of Engineering Gifu University
関連論文
- Bioconversion of 1-adamantanol to 1,3-adamantanediol using Streptomyces sp. SA8 oxidation system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Purification, Characterization and Gene Cloning of Thermostable Ο-Acetyl-L-Homoserine Sulfhydrylase Forming γ-Cyano-α-Aminobutyric Acid (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification, Characterization and Gene Cloning of Thermostable ο-Acetyl-L-Serine Sulfhydrylase Forming β-Cyano-L-Alanine
- Occurrence of Amidases in the Industrial Microbe Rhodococcus rhodochrous J1
- Purification and Characterization of Vanillyl-Alcohol Oxidase from Byssochlamys fulva V107
- Isolation and Identification of an Iron-Oxidizing Bacterium Which Can Grow on Tetrathionate Medium and the Properties of a Tetrathionate-Decomposing Enzyme Isolated from the Bacterium
- Purification and Some Properties of a Tetrathionate Decomposing Enzyme from Thiobacillus thiooxidans
- TYPE AND INCIDENCE OF VENTRICULAR PREMATURE BEATS USING THE FRANK ORTHOGONAL LEADS : Electrocardiography・Vectorcardiography II : PROCEEDINGS OF THE 44th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Bioelectrochemically Accelerated Microbial Conversion of Nicotinic Acid to 6-Hydroxynicotinic Acid on Microorganism-immobilized Column Electrolytic Flow System
- Secretion Mode and Subcellular Localization of Human Interferon-β Exogenously Expressed in Porcine Renal Epithelial LLC-PK_1 Cells(Biopharmacy)
- Novel Reversible Indole-3-carboxylate Decarboxylase Catalyzing Nonoxidative Decarboxylation(Microbiology & Fermentation Technology)
- Synthesis of D-Cysteine from a Racemate of 3-Chloroalanine by Phenylhydrazine-treated Cells of Pseudomonas putida CR 1-1
- CoASAc-independent Phosphoenolpyruvate Carboxylase from an Obligate Methylotroph Hyphomicrobium methylovorum GM2 : Purification and Characterization
- Microbial HydroxyIation of 3-Cyanopyridine to 3-Cyano-6-hydroxypyridine
- Isolation of Advantageous Mutants of Pseudomonas chlororaphis B23 for the Enzymatic Production of Acrylamide(Microbilolgy & Fermentation Industry)
- Optimum Culture Conditions for Production by Pseudomonas chlororaphis B23 of Nitrile Hydratase(Microbiology & Fermentaion Industry)
- Efficient Synthesis of Enantiopure 1,2-Bis (hydroxymethyl)-3,3-difluorocyclopropane Derivatives through Lipase-Catalyzed Reaction
- Transplantation of the urinary bladder and other organs in the subcutaneous tissue induces cyst formation and epithelialization : its potential usefulness in regenerative medicine
- An Assay for Serine-glyoxylate Aminotransferase(Microbiology & Fermentation Industry)
- Ferulic Acid Production from Clove Oil by Pseudomonas fluorescens E118
- Conversion of Isoeugenol into Vanillic Acid by Pseudomonas putida I58 Cells Exhibiting High Isoeugenol-Degrading Activity
- Microbial Synthesis of Coniferyl Alcohol by the Fungus Byssochlamys fulva V107
- Efficient L-Serine Production from Methanol and Glycine by Resting Cells of Methylobacterium sp. Strain MN43
- L-Serine Synthesis Using the Resting Hyphomicrobium sp. NCIB10099 Cell under Suppressive Conditions for L-Serine-Degrading Activity
- L-Serine Production Using a Resting Cell System of Hyphomicrobium Strains
- Isolation of a Thiamine-binding Protein from Rice Germ and Distribution of Similar Proteins
- Enzymatic Functionalization of Aromatic N-Heterocycles : Hydroxylation and Carboxylation
- Purification and Characterization of Nicotinic Acid Dehydrogenase from Pseudomonas fluorescens TN5
- Microbial Production of 6-Hydroxynicotinic Acid, an Important Building Block for the Synthesis of Modern Insecticides
- Production of 6-Hydroxynicotinic Acid from Nicotinic Acid by Resting Cells of Pseudomonas fluorescens TN5
- Purification and Characterization of a Novel (R)-Imine Reductase from Streptomyces sp. GF3587
- Evaluation of Brittleness of Porcelain Fused to Pure Titanium by Fracture Toughness, Hardness and Fracture Energy
- Thiamin-Binding Protein from Sunflower Seeds
- Characterization of Thiamin-Binding Protein from Buckwheat Seeds
- Purification and Characterization of an ε-Poly-L-Lysine-Degrading Enzyme from the ε-Poly-L-Lysine-Tolerant Chryseobacterium sp. OJ7 (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification and Characterization of a Novel (R)-Imine Reductase from Streptomyces sp. GF3587
- Molecular and Catalytic Properties of 2,4′-Dihydroxyacetophenone Dioxygenase from Burkholderia sp. AZ11
- Molecular and Catalytic Properties of 2,4'-Dihydroxyacetophenone Dioxygenase from Burkholderia sp. AZ11
- Oxidative Degradation of 4-Hydroxyacetophenone in Arthrobacter sp. TGJ4
- Oxidation of aromatic N-heterocyclic compounds to N-oxides by Verticillium sp. GF39 cells(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Enzymatic Synthesis of D-Cysteine by 3-Chloro-D-alanine Resistant Pseudomonas putida CR 1-1
- Syntheses of S-Substituted L-Homocysteine Derivatives by Cystathionine γ-Lyase of Streptomyces phaeochromogenes
- Structures of Rhodonocardins Produced by a Nocardia sp.