Secretion Mode and Subcellular Localization of Human Interferon-β Exogenously Expressed in Porcine Renal Epithelial LLC-PK_1 Cells(Biopharmacy)

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概要

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• We have investigated the secretion polarity of human interferon-β (HuIFN-β) exogenously expressed in the porcine renal proximal tubule cell line, LLC-PK_1. In these cells, stably expressed HuIFN-β was secreted to the apical and basolateral sides. However, when transiently expressed by apical lipofection, HuIFN-β was secreted to both cell sides, while basolateral-preferential secretion was seen for basal transfection. Confocal imaging using enhanced green fluorescent protein (EGFP)-tagged HuIFN-β revealed no difference in the subcellular distribution in either of the chimeric protein-expressing cells examined. These results suggest that the secretion polarity of HuIFN-β is regulated by a post frans-Golgi network in a cell type-dependent manner.

• 公益社団法人日本薬学会の論文
• 2004-10-01

著者

• Yoshida Toyokazu

  Department of Biomolecular Science, Gifu University

• Watanabe Yoshihiko

  Department of Internal Medicine, Fujita-Gakuen Health University School of Medicine

• NISHIKAWA Makiya

  Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University

• Watanabe Y

  Department Of Molecular Biopharmacy And Genetics Graduate School Of Pharmaceutical Sciences Tohoku U

• TAKAKURA Yoshinobu

  Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University

• Maruyama Masato

  Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyot

• NISHIO Teruko

  Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyot

• Yoshida Toyokazu

  Department Of Biomolecular Science Gifu University

• Yoshida Toyokazu

  Department Of Biopharmaceutics And Drug Metabolism Graduate School Of Pharmaceutical Sciences Kyoto

• Nishikawa Makiya

  Department Of Biopharmaceutics And Drug Metabolism Graduate School Of Pharmaceutical Science Kyoto U

• Takakura Yoshinobu

  Department Of Basic Pharmaceutics Faculty Of Pharmaceutical Sciences Kyoto University

• Takakura Yoshinobu

  Department Of Drug Delivery Research Graduate School Of Pharmaceutical Sciences Kyoto University

• Watanabe Yoshifumi

  Department Of Biomolecular Engineering Tokyo Institute Of Technology

• Watanabe Yoshihiko

  Department Of Anesthesia Tokyo Metropolitan Hiroo General Hospital

• Nishio T

  Department Of Biopharmaceutics And Drug Metabolism Graduate School Of Pharmaceutical Sciences Kyoto

• Nishikawa M

  Department Of Biopharmaceutics And Drug Metabolism Graduate School Of Pharmaceutical Sciences Kyoto

• Yoshida Toyokazu

  Department Of Biomolecular Science Faculty Of Engineering Gifu University

• Maruyama Masato

  Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University

• Watanabe Yoshihiko

  Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University

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