Exogenous Expression of Interferon-β in Cultured Brain Microvessel Endothelial Cells(Biochemistry/Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Brain microvessel endothelial cells (BMECs) make up the blood-brain barrier (BBB) and regulate the passage of therapeutic proteins as well as drugs from the cerebrovasucular circulation to the brain. In the present study, we transferred mouse or human interferon-β (IFN-β) gene via cationic liposomes into primary cultures of bovine BMECs developed as an in vitro model of the BBB. The gene-transferred BMECs secreted transiently a substantial amount of IFN activity more efficiently during the growth phase than at confluence. This was suggested to be due to a difference in the potential for plasmid incorporation between growing and confluent BMECs in a series of cell association experiments with ^<32>P-labelled plasmid DNA. Furthermore, when BMEC monolayers in Transwell plates were transfected with the IFN-β-expression vectors from the upper side, IFN-β was predominantly detected in the upper compartments, suggesting polarized secretion of the transgene products in BMEC monolayers. These findings provide important basic information about therapeutic secretory protein gene delivery to BMECs.
- 公益社団法人日本薬学会の論文
- 2004-09-01
著者
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Yamashita Fumiyoshi
第一三共 葛西研究開発センター
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Yamashita Fumiyoshi
Department Of Drug Delivery Research Graduate School Of Pharmaceutical Sciences Kyoto University
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Yamashita Fumiyoshi
Departments Of Drug Delivery Research Graduate School Of Pharmaceutical Sciences Kyoto University
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Yamashita Fumiyoshi
Graduate School Of Pharmaceutical Sciences Kyoto University
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Hashida Mitsuru
第一三共 葛西研究開発センター
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Hashida Mitsuru
Department Of Basic Pharmaceutics Faculty Of Pharmaceutical Sciences Kyoto University
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Hashida Mitsuru
京都大学 薬学研究科病態情報薬学
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Hashida Mitsuru
Dep. Of Drug Delivery Res. Graduate School Of Pharmaceutical Sciences Kyoto Univ. 46-29 Yoshidashimo
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Hashida Mitsuru
Inst. Of Integrated Cell-material Sciences (icems) Kyoto Univ.
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Yamashita Fumiyoshi
Dep. Of Drug Delivery Res. Graduate School Of Pharmaceutical Sciences Kyoto Univ. 46-29 Yoshidashimo
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Watanabe Yoshihiko
Department of Internal Medicine, Fujita-Gakuen Health University School of Medicine
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NAKAMURA Masaru
Department of Computer Science and Engineering, Nagoya Institute of Technology
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Watanabe Y
Department Of Molecular Biopharmacy And Genetics Graduate School Of Pharmaceutical Sciences Tohoku U
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Hashida M
Department Of Drug Delivery Research Graduate School Of Pharmaceutical Sciences Kyoto University
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Hashida Mitsuru
Graduate School Of Pharmaceutical Sciences Kyoto University
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TAKAKURA Yoshinobu
Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University
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YAMASHITA Fumiyoshi
京大院薬
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HASHIDA Mitsuru
京大院薬
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YAMAGUCHI Shinsuke
Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University
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Yamaguchi Shinsuke
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Yamaguchi Shinsuke
Department Of Drug Delivery Research Graduate School Of Pharmaceutical Sciences Kyoto University
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Takakura Yoshinobu
Department Of Basic Pharmaceutics Faculty Of Pharmaceutical Sciences Kyoto University
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Takakura Yoshinobu
Department Of Drug Delivery Research Graduate School Of Pharmaceutical Sciences Kyoto University
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Watanabe Yoshifumi
Department Of Biomolecular Engineering Tokyo Institute Of Technology
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Watanabe Yoshihiko
Department Of Anesthesia Tokyo Metropolitan Hiroo General Hospital
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Yamashita Fumiyoshi
Dep. Of Drug Delivery Res. Graduate School Of Pharmaceutical Sciences Kyoto Univ.
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Nakamura Masaru
Department Of Computer Science And Engineering Nagoya Institute Of Technology
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Nakamura Masaru
Department Of Basic Life Science Faculty Of Medicine Teikyo University
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Nakamura Masaru
Department Of Applied Chemistry Faculty Of Science And Engineering Kinki University
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Watanabe Yoshihiko
Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University
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Nakamura Masaru
Departments of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University
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