Expression and Visualization of a Human Interferon-β-Enhanced Green Fluorescent Protein Chimeric Molecule in Cultured Cells (Biochemistry/Molecular Biology)
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概要
- 論文の詳細を見る
We have constructed a recombinant cDNA encoding the chimeric protein between human IFN-β (HuIFN-β) and enhanced green fluorescent protein (EGFP) to elucidate the intracellular localization of IFN-β. Transient expression of the chimeric molecule, HuIFN-β-EGFP, in L cells demonstrated that the chimeric molecule secreted from the cells had an intact biological activity as far as antiviral effect was concerned. Immunostaining of the transfected cells using anti-HuIFN-β antibody demonstrated that green-fluorescence was co-localized with the IFN signal and its profile was similar to IFN signals in the cells transfected with HuIFN-β expressing plasmid DNA. These results indicate that the HuIFN-β-EGFP chimeric gene was expressed as a chimeric protein and the chimera was transported via the regular secretory pathway in the cells. In other cell types, the fluorescence derived from the chimeric protein was also seen on cytoplasmic vesicular structures. These results suggest that HuIFN-β-EGFP will be a useful tool to investigate the intracellular trafficking processes of HuIFN-β in a variety of cell types.
- 公益社団法人日本薬学会の論文
- 2004-03-01
著者
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Yamashita Fumiyoshi
第一三共 葛西研究開発センター
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Yamashita Fumiyoshi
Department Of Drug Delivery Research Graduate School Of Pharmaceutical Sciences Kyoto University
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Yamashita Fumiyoshi
Departments Of Drug Delivery Research Graduate School Of Pharmaceutical Sciences Kyoto University
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Yamashita Fumiyoshi
Graduate School Of Pharmaceutical Sciences Kyoto University
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Hashida Mitsuru
第一三共 葛西研究開発センター
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Hashida Mitsuru
Department Of Basic Pharmaceutics Faculty Of Pharmaceutical Sciences Kyoto University
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Hashida Mitsuru
京都大学 薬学研究科病態情報薬学
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Hashida Mitsuru
Dep. Of Drug Delivery Res. Graduate School Of Pharmaceutical Sciences Kyoto Univ. 46-29 Yoshidashimo
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Hashida Mitsuru
Inst. Of Integrated Cell-material Sciences (icems) Kyoto Univ.
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Yamashita Fumiyoshi
Dep. Of Drug Delivery Res. Graduate School Of Pharmaceutical Sciences Kyoto Univ. 46-29 Yoshidashimo
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NAKAMURA Masaru
Department of Computer Science and Engineering, Nagoya Institute of Technology
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Watanabe Y
Department Of Molecular Biopharmacy And Genetics Graduate School Of Pharmaceutical Sciences Tohoku U
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Hashida M
Department Of Drug Delivery Research Graduate School Of Pharmaceutical Sciences Kyoto University
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Hashida Mitsuru
Graduate School Of Pharmaceutical Sciences Kyoto University
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YAMASHITA Fumiyoshi
京大院薬
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HASHIDA Mitsuru
京大院薬
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MARUYAMA Masato
Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University
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TAKAKURA Yoshinobu
Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University
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WATANABE Yoshihiko
Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Kyoto University
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Takakura Yoshinobu
Department Of Basic Pharmaceutics Faculty Of Pharmaceutical Sciences Kyoto University
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Takakura Yoshinobu
Department Of Drug Delivery Research Graduate School Of Pharmaceutical Sciences Kyoto University
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Watanabe Yoshifumi
Department Of Biomolecular Engineering Tokyo Institute Of Technology
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Yamashita Fumiyoshi
Dep. Of Drug Delivery Res. Graduate School Of Pharmaceutical Sciences Kyoto Univ.
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Nakamura Masaru
Department Of Computer Science And Engineering Nagoya Institute Of Technology
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Nakamura Masaru
Department Of Basic Life Science Faculty Of Medicine Teikyo University
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Nakamura Masaru
Department Of Applied Chemistry Faculty Of Science And Engineering Kinki University
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Maruyama Masato
Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University
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Watanabe Yoshihiko
Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University
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Nakamura Masaru
Departments of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University
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