Fish 3β-Hydroxysteroid Dehydrogenase/Δ^5-Δ^4 Isomerase : Antibody Production and Their Use for the Immunohistochemical Detection of Fish Steroidogenic Tissues
スポンサーリンク
概要
- 論文の詳細を見る
We have produced polyclonal antibodies against two oligopeptides corresponding to middle and c-terminal regions of amino acid sequences predicted from rainbow trout (Oncorhynchus mykiss) 3β-hydroxysteroid dehydrogenase (3β-HSD) cDNA (Sakai et al., 1994, FEBS Letters 350, 309-313). Both antibodies (α-tr3β-M and α-tr3β-C) recognized recombinant rainbow trout 3β-HSD protein derived from rabbit reticulocyte lysate system and non-steroidogenic mammalian COS-1 cell lysate. Immunoblot analysis of rainbow trout ovarian follicle homogenates revealed specific recognition of 3β-HSD protein. In rainbow trout testis, furthermore, immunoreactive 3β-HSD localized in Leydig cells in the interstitium of immature testes and interrenal cells in the head kidney. These results indicate that both α-3β-HSD antibodies recognized rainbow trout 3β-HSD protein at the level of immunoblot and immunohistochemical analyses. Furthermore, both antibodies also recognized immunohistochemically 3β-HSD in various steroidogenic organs (ovary, testis, and interrenal glands) of several teleost fishes.
- 社団法人日本動物学会の論文
- 1996-12-15
著者
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小林 亨
Sesoko Station Tropical Biosphere Research Center University Of Ryukyus
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NAKAMURA Masaru
Department of Computer Science and Engineering, Nagoya Institute of Technology
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NAGAHAMA Yoshitaka
Laboratory of Reproductive Biology, National Institute for Basic Biology
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小林 亨
岡崎国立共同研究機構・基礎生物学研究所・生殖研究部門
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Kobayashi Tohru
Laboratory of Reproductive Biology, National Institute for Basic Biology
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Kusama Takehiko
Department Of Aquatic Biosciences Tokyo University Of Fisheries
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中村 將
Sesoko Station Tropical Biosphere Research Center University Of Ryukyus
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Kajiura Hiroko
Laboratory Of Reproductive Biology National Institute For Basic Biology
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Chang X.-T.
Laboratory of Reproductive Biology National Institute for Basic Biology
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Chang X.-t.
Laboratory Of Reproductive Biology National Institute For Basic Biology:molecular Biomechanics Schoo
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Kajiura‐kobayashi H
Laboratory Of Reproductive Biology National Institute For Basic Biology
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Nagahama Yoshitaka
Laboratory Of Reproduction National Institute For Basic Biology
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Kobayashi Tohru
Laboratory Of Reproduction National Institute For Basic Biology
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Nakamura Masaru
Department Of Basic Life Science Faculty Of Medicine Teikyo University
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Nakamura Masaru
Department Of Applied Chemistry Faculty Of Science And Engineering Kinki University
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Kajiura Hiroko
Laboratory of Analytical Chemistry, Faculty of Science, Nagoya University
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