Characterization of Two Types of Cytochrome P450 Aromatase in the Serial-sex Changing Gobiid Fish, Trimma okinawae
スポンサーリンク
概要
- 論文の詳細を見る
To investigate the role of estrogen in the serial-sex changing fish Trimma okinawae, we isolated complementary DNAs encoding two distinct cytochrome P450 aromatase isoforms from adult ovary and brain (termed P450aromA and P450aromB, respectively). Sequence and phylogenic analyses showed that the goby P450arom forms belong to two separate CYP19 subfamilies. Transient expression of these cDNAs in HEK293 cells caused conversion of exogenous testosterone to estradiol-17β. RT-PCR showed that P450aromA was expressed in the brain, spleen, testis and ovary. P450aromB was expressed in the brain, liver, testis and ovary. In situ hybridization studies showed that P450aromA mRNA, but not P450aromB mRNA, was present in both ovary and testis. Positive signals were restricted to granulosa cells of vitellogenic follicles and interstitial cells of mature testis. Ovarian expression of both P450arom genes during the spawning cycle was examined by quantitative real-time RT-PCR. P450aromA transcripts increased during vitellogenesis and decreased prior to spawning. In contrast, P450aromB transcripts were barely detectable and did not correlate with ovarian development. These findings suggest that P450aromA, but not P450aromB, is involved in regulating ovarian vitellogenesis in goby.
- 社団法人日本動物学会の論文
- 2004-04-25
著者
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小林 亨
Sesoko Station Tropical Biosphere Research Center University Of Ryukyus
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Suzuki Norio
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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小林 亨
Department Of Regulation Biology Faculty Of Science Saitama University
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SUZUKI Nobuo
Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University
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NAGAHAMA Yoshitaka
Laboratory of Reproductive Biology, National Institute for Basic Biology
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Shimizu Nobuaki
Institute Of Nature And Environmental Technology Kanazawa University
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Nakamura Masaru
Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus
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小林 亨
岡崎国立共同研究機構・基礎生物学研究所・生殖研究部門
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Suzuki N
Hokkaido Univ. Sapporo Jpn
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Suzuki N
Noto Marine Laboratory Institute Of Nature And Environmental Technology Kanazawa University
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Nagahama Yoshitaka
National Institute for Basic Biology
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Kobayashi Tohru
Laboratory of Reproductive Biology, National Institute for Basic Biology
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Suzuki N
Noto Marine Laboratory Faculty Of Science Kanazawa University
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Kusama Takehiko
Department Of Aquatic Biosciences Tokyo University Of Fisheries
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中村 將
Sesoko Station Tropical Biosphere Research Center University Of Ryukyus
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E. Craig
Laboratory Of Reproductive Biology National Institute For Basic Biology
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SUNOBE Tomoki
Natural History Museum and Institute
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Kobayashi Yasuhisa
Division of Biological Science, Graduate School of Science, Hokkaido University
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Morrey Craig
Laboratory of Reproductive Biology, National Institute for Basic Biology
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Nagahama Yoshitaka
Department Of Molecular Biomechanics The Graduate University For Advanced Studies:laboratory Of Repr
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Shimizu Nobuyoshi
Advanced Research Center For Genome Super Power Keio University
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Morrey C
Laboratory Of Reproductive Biology National Institute For Basic Biology
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Morrey Craig
Laboratory Of Reproductive Biology National Institute For Basic Biology
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Nagahama Y
National Institute For Basic Biology
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Nagahama Yoshitaka
Laboratory Of Reproduction National Institute For Basic Biology
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Sunobe T
Tateyama Station Field Science Center Tokyo University Of Marine Science And Technology
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Nakamura Masaru
Sesoko Station Tropical Biosphere Research Center University Of The Ryukyus
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Kobayashi Tohru
Laboratory Of Reproduction National Institute For Basic Biology
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Suzuki Norio
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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