Field Survey of Sex-Reversals in the Medaka, Oryzias latipes : Genotypic Sexing of Wild Populations(Genetics)
スポンサーリンク
概要
- 論文の詳細を見る
The medaka, Oryzias latipes, has an XX/XY sex determination mechanism. A Y-linked DM domain gene, DMY, has been isolated by positional cloning as a prime candidate for the sex-determining gene. Furthermore, the crucial role of DMY during male development was established by studying two wild-derived XY female mutants. In this study, to find new DMYand sex-determination related gene mutations, we conducted a broad survey of the genotypic sex (DMY-negative or DMY-positive) of wild fish. We examined 2274 wild-caught fish from 40 localities throughout Japan, and 730 fish from 69 wild stocks from Japan, Korea, China, and Taiwan. The phenotypic sex type agreed with the genotypic sex of most fish, while 26 DMY-positive (XY) females and 15 DMY-negative (XX) males were found from 13 and 8 localities, respectively. Sixteen XY sex-reversals from 11 localities were mated with XY males of inbred strains, and the genotypic and phenotypic sexes of the F_1 progeny were analyzed. All these XY sex-reversals produced XY females in the F_1 generation, and all F_1 XY females had the maternal Y chromosome. These results show that DMY is a common sex-determining gene in wild populations of O. latipes and that all XY sex-reversals investigated had a DMY or DMY-linked gene mutation.
- 社団法人日本動物学会の論文
- 2004-06-25
著者
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浜口 智志
大阪大学大学院工学研究科原子分子イオン制御理工学センター
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小林 亨
Department Of Regulation Biology Faculty Of Science Saitama University
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HAMAGUCHI Satoshi
Graduate School of Energy Science, Kyoto University
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小林 亨
岡崎国立共同研究機構・基礎生物学研究所・生殖研究部門
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Sakaizumi Mitsuru
Graduate School of Science and Technology, Niigata University
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Hamaguchi Satoshi
Niigata Univ. Niigata Jpn
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Hamaguchi S
Niigata Univ. Niigata Jpn
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Hamaguchi Satoshi
Environmental Science And Technology Graduate School Of Science And Technology Niigata University
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Hamaguchi S.
Dept. Of Environ. Sci. Fac. Of Sci. Niigata Univ.
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Sakaizumi M
Dep. Of Environmental Sci. Fac. Of Sci. Niigata Univ.
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Sakaizumi Mitsuru
Environmental Science And Technology Graduate School Of Science And Technology Niigata University
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Sakaizumi Mitsuru
Graduate School Of Science And Technology Niigata University
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Sakaizumi Mitsuru
Graduate School Of Science And Technology Niigata University:department Of Environmental Science Fac
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Otake Hiroyuki
Graduate School of Science and Technology, Niigata University
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Shinomiya Ai
Department of Environmental Science, Faculty of Science, Niigata University
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Shinomiya Ai
Graduate School of Science and Technology, Niigata University
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Kusama Takehiko
Department Of Aquatic Biosciences Tokyo University Of Fisheries
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Togashi Ken-ichi
Graduate School Of Science Technology Niigata University
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Ohtake H
Graduate School Of Science And Technology Niigata University
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Shinomiya A.
Graduate School Of Science And Technology Niigata University
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Hamaguchi Satoshi
Graduate School Of Energy Science Kyoto University
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