Isolation of a sex chromosome-specific DNA sequence in the medaka, Oryzias latipes
スポンサーリンク
概要
- 論文の詳細を見る
In the medaka, Oryzias latipes, which does not have cytologically recognizable sex chromosomes, the mechanism of sex determination (XX/XY) can be revealed by genetic crosses using a particular pigment gene. Since the only known sex-linked marker is this pigment gene, little information is available on the genetic maps of the medakas sex chromosomes. High resolution genetic maps of its sex chromosomes are necessary to hunt for the sex-determining factor by the positional cloning method. In the present study, we isolated a sex-linked marker using the genomic differences between inbred strains of medaka. We established a congenic strain whose sex-determining region is derived from the HNI strain and whose genetic background is derived from the Hd-rR strain. Using differences between the genomes of the congenic and Hd-rR strains, we isolated a sex-linked clone (pHO5.5) from a random genomic library constructed from the HO5 strain of medaka. The pHO5.5-related sequences were conserved among Oryzias species. A linkage analysis using backcross progeny from the Hd-rR and HNI strains demonstrated perfect linkage between the pHO5.5-related sequence and the sex. We, hence, designated the locus of the pHO5.5-related sequence on sex chromosomes of medaka as Sex-Linked 1 (SL1). Using this sequence, we could identify the sex of inbred strains of medaka by PCR or Southern blotting. This sequence may be useful for genetic mapping on the sex chromosomes. We also discuss the availability of the congenic strain established in this study for isolating other sex-linked clones.
- 日本遺伝学会の論文
著者
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SAKAIZUMI Mitsuru
Department of Environmental Science, Faculty of Science, Niigata University
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Kobayashi Tetsuya
Laboratory Of Integrative Brain Sciences Department Of Biology Faculty Of Education And Integrated A
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Matsuda Masaru
Laboratory of Reproductive Biology, National Institute for Basic Biology
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Hamaguchi Satoshi
Department of Biology, College of General Education, Niigata University
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Matsuda M
Laboratory Of Reproductive Biology National Institute For Basic Biology
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Oshiro Takashi
Department Of Aquatic Biosciences Tokyo University Of Fisheries
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小林 亨
岡崎国立共同研究機構・基礎生物学研究所・生殖研究部門
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Oshiro T
Department Of Aquatic Biosciences Tokyo University Of Fisheries
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Hamaguchi S.
Dept. Of Environ. Sci. Fac. Of Sci. Niigata Univ.
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Matsuda Masaru
Graduate School of Science and Technology, Niigata University
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KURIHARA Yasuyuki
Department of Environment and Natural Sciences, Graduate School of Environment and Information Scien
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Matsuda Masaru
Department Of Aquatic Bioscience Tokyo University Of Fisheries
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Sakaizumi M
Dep. Of Environmental Sci. Fac. Of Sci. Niigata Univ.
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Sakaizumi Mitsuru
Department Of Biology College Of General Education Niigata University
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Hamaguchi Satoshi
Department Of Biology College Of General Education Niigata University
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KUSAMA Takehiko
Department of Aquatic Biosciences, Tokyo University of Fisheries
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Kusama Takehiko
Department Of Aquatic Biosciences Tokyo University Of Fisheries
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KURIHARA Yasuyuki
Faculty of Engineering, Yokohama National University
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Togashi Ken-ichi
Graduate School Of Science Technology Niigata University
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Kobayashi Tetsuya
Deparatment Of Regulation Biology Faculty Of Science Saitama University
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Matsuda Masaru
Graduate School Of Engineering Hokkaido University
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Kurihara Y
Faculty Of Engineering Yokohama National University
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Kurihara Yasuyuki
Department Of Environment And Natural Sciences Graduate School Of Environment And Information Scienc
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OSHIRO Takashi
Department of Agricultural Chemistry, Kyoto University
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