Distribution of Actin and Tubulin in C6 Glioma Cells during Arborization Induced by Cytochalasin D
スポンサーリンク
概要
- 論文の詳細を見る
C6 glioma cells were arborized by cytochalasin D (2μM), while the processes induced by the drug were destroyed by colcemid (2 μM). Distribution of actin and tubulin during the arborization was investigated by fluorescence microscopy to identify core structures involved in arborization. There were two types of actin filaments in C6 cells : one was oriented radially and the other was concentric. Cytochalasin D destroyed the actin filaments in sequence and transiently left a radial structure in the early stage (the first 3 min) of arborization. Microtubules were also organized similarly. At a later stage (15 min), few radial actin filaments remained, whereas microtubule bundling proceeded and seemed to support a starfish-like cell morphology. In the last stage (2 hr), most actin-staining was found around the plasma membrane undercoat area, whereas the microtubules were bundled in the core part of the processes. Radial actin filaments, which showed higher stability, seemed to be important collectively in the first step of arborization as a transient organizer of the cytoskeleton. Distribution of α-actin and vinculin on actin filaments was investigated as potential factors stabilizing the radial filaments because of their affinity for the barbed ends of actin filaments for which cytochalasin D also had affinity. Results showed that α-actinin and vinculin were preferentially located on the radial filaments rather than on the concentric filaments.
- 社団法人日本動物学会の論文
著者
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Kobayashi Tetsuya
Laboratory Of Integrative Brain Sciences Department Of Biology Faculty Of Education And Integrated A
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KOBAYASHI TAKAAKI
Department of Environmental Chemistry and Engineering, Tokyo Institute of Technology
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Kobayashi Takaaki
Department Of Applied Immunology Nagoya University School Of Medicine
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Kobayashi Takaaki
Department Of Biochemistry (i) Jikei University School Of Medicine
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