Sodium Fluoride (NaF) Releases the Two-Cell Block in Mouse Embryos
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概要
- 論文の詳細を見る
This study was undertaken to elucidate the mechanism underlying the inability of mouse embryos to develop in culture past the 2-cell stage (2-cell block). In complete TLP-PVA medium, the rate of development of non-inbred (ICR) mouse 2-cell embryos to 3- and 4-cell stages (≧3 cells) was only 11%. However, sodium fluoride (NaF, an inhibitor of enolase in glycolysis) significantly improved the rate of further development of 2-cell embryos in a dose-fashion manner. NaF at 5 mM allowed 54% of 2-cell embryos to develop to ≧3 cells, although NaF at concentrations greater than 7.5 mM stunted their development. The removal of glucose and phosphate from the TLP-PVA medium improved the rate of development of 2-cell embryos to ≧3 cell(42%), but the rate of development of 2-cell embryos to ≧3 cells was enhanced (77%) when NaF at 2.5 mM was added to the medium without glucose and phosphate. The effect of NaF at 2.5 mM on ^<14>CO_2 and [^<14>C]lactate production from [^<14>C]glucose was also examined. NaF at 2.5 mM significantly inhibited both ^<14>CO_2 and [^<14>C]lactate production from [6-^<14>C]glucose, but not ^<14>CO_2 production from [1-^<14>C]glucose, although it had no effect on the uptake of [^3H]deoxy-D-glucose (a non-metabolizable analogue of glucose) by the embryos. When the intracellular level of adenosine triphosphate (ATP) during early development of mouse embryos was examined, it was higher before rather than after cleavage. In addition, the ATP level was much higher before the 2nd cleavage 30 hr after insemination in unblocked embryos than that in blocked ones. These results suggest that the flow rate of glucose in glycolysis may regulate the development of 2-cell embryos to ≧3 cells, and that the intracellular level of ATP may. be critical to early phase of mouse embryo development in vitro.
- 社団法人日本動物学会の論文
- 1993-08-16
著者
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Kobayashi Tetsuya
Laboratory Of Integrative Brain Sciences Department Of Biology Faculty Of Education And Integrated A
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NAKAMURA Masahisa
Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University
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Nakamura Masahisa
Waseda Univ. Tokyo Jpn
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Okinaga Shoichi
Department of Obstetrics & Gynecology, Teikyou University, School of Medicine, Ichihara Hospital,
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KOBAYASHI Takuro
Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu Univers
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Nakamura M
Hiroshima Univ. Hiroshima Jpn
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Nakamura Masahisa
Department Of Biology Faculty Of Education And Integrated Arts And Sciences Waseda University
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Nakamura Masahisa
Department Of Biology Faculty If Education And Integrated Arts And Sciences Waseda University
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Aoki Keiji
Department of Biology, School of Education, Waseda University
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Aoki Keiji
Department Of Biology School Of Education Waseda University
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Kobayashi Takuro
Department Of Obstetrics And Gynecology School Of Medicine Teikyo University
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Okinaga Shoichi
Department Of Obstetrics And Gynecology School Of Medicine Teikyo University
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Kobayashi Takuro
Department Of Dermatology Kagoshima University Graduate School Of Medical And Dental Sciences
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Kobayashi Takuro
Department Of Dermatology Field Of Sensory Organology Kagoshima University Graduate School Of Medica
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