A mRNA for Membrane Form of Guanylyl Cyclase Is Expressed Exclusively in the Testis of the Sea Urchin Hemicentrotus pulcherrimus
スポンサーリンク
概要
- 論文の詳細を見る
A cDNA clone encoding the membrane form of guanylyl cyclase was isolated from a Hemicentrotus pulcherrimus testis cDNA library and its nucleotide sequence was determined. The cDNA was 4123 bp long and an open reading frame predicted a protein of 1125 amino acids including an apparent signal peptide of 21 residues; a single transmembrane domain of 25 amino acids which divides the mature protein into an amino-terminal, extracellular domain of 485 amino acids and a carboxyl-teninal, intracellular domain of 594 amino acids. Three potential N-linked glycosylation sites were present in the extracellular domain. Northern blot analysis of poly(A)+RNA from testes, ovaries, eggs and embryos at various developmental stages showed that the cDNA encoding guanylyl cyclase hybridized to a mRNA of 4.4 kb from the testes.We developed a large scale purification method of the phosphorylated (131 kDa) and dephosphorylated (128 kDa) forms of the membrane-bound guanylyl cyclase from H. pulcherrimus spermatozoa. The purified 131 kDa and 128 kDa forms of the guanylyl cyclase contained 26.0 ±1.3 and 4.3 ±0.7 moles of phosphate per mot protein (mean ±S.D.; n=6), respectively. The amino-terminal amino acids of both the 131 kDa and 128 kDa forms of the guanylyl cyclase could not be detected, suggesting that they were blocked.
- 社団法人日本動物学会の論文
- 1996-04-15
著者
-
NOMURA Kohji
Department of Geriatric Medicine, Kanazawa Medical University
-
Suzuki Norio
Division of Biological Sciences, Graduate School of Science, Hokkaido University
-
Nomura K
Department Of Biochemistry Tokyo Metropolitan Institute Of Gerontology
-
Shimizu Takeshi
Division of Cardiology, Aichi Prefectural Cardiovascular and Respiratory Center
-
Suzuki N
Toshiba Corp. Kawasaki Jpn
-
Suzuki N
Hokkaido Univ. Sapporo Jpn
-
Nomura Kohji
Department Of Biochemistry Tokyo Metropolitan Institute Of Gerontology
-
Suzuki N
Noto Marine Laboratory Faculty Of Science Kanazawa University
-
Furuya H
Osaka Univ. Osaka Jpn
-
Furuya Hirotaka
Division of Biological Sciences, Graduate School of Science, Hokkaido University
-
Takeda Kenji
Division of Biological Sciences, Graduate School of Science, Hokkaido University
-
Hoshino Katsuaki
Division of Biological Sciences, Graduate School of Science, Hokkaido University
-
Suzuki N
Department Of Surgery Gunma Children's Medial Center
-
Hoshino Katsuaki
Division Of Biological Sciences Graduate School Of Science Hokkaido University
-
Takeda Kenji
Division Of Biological Sciences Graduate School Of Science Hokkaido University
-
Shimizu T
Section Of Biotechnology Department Of Applied Technology Hokkaido Food Processing Research Center:(
-
Suzuki Norio
Division Of Biological Sciences Graduate School Of Science Hokkaido University
関連論文
- Tilting-Induced Decrease in Systolic Blood Pressure in Bedridden Hypertensive Elderly Inpatients : Effects of Azelnidipine
- Extract from Inflamed Skin of Rabbits Increases Enkephalin Concentrations in Cerebrospinal Fluid in Rats
- Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes
- 4-2 阪大基礎工学部における『PBL』科目の実践(第4分科会:「物理教育での課題研究」,分科会)
- 近畿支部特集1 座談会「21世紀の子どもたちに物理教育は必要か」
- 21世紀の子どもたちに物理教育は必要か
- 鉄の超伝導
- 圧力誘起超伝導 : 分子性結晶を中心に
- Application of Frozen-Phonon Method to Lattice Dynamics in FCC Solid Iodine
- PJ-784 The Greater Efficacy of Extensive Encircling Pulmonary Vein Isolation for Persistent Atrial Fibrillation Than Individual Pulmonary Vein Antrum Isolation(Arrhythmia, therapy(17)(A),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the J
- PJ-599 Efficacy of Pulmonary Vein Isolation in Patients with Bradycardia-Tachycardia Syndrome(Arrhythmia, therapy(16)(A),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-394 Sleep Apnea Syndrome May Play a Role in the Higher Incidence of Non-Pulmonary Vein Ectopic Beats Initiating Atrial Fibrillation(Arrhythmia, diagnosis/Pathophysiology/EPS(14)(A),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japa
- PJ-284 Behavior of Atrial Premature Beats Triggering the Recurrences of Atrial Fibrillation After Pulmonary Vein Isolation(ECG/Body surface potential mapping/Holter(05)(A),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circula
- Role of Boundary Plasma in Lower-Hybrid-Frequency Heating of a Tokamak
- Loss Mechanism of Energetic Ions Produced during the Lower Hybrid Heating of JFT-2 Tokamak
- Parametric Heating by Radio-Frequency near the Lower Hybrid Frequency in the JFT-2 Tokamak
- Impurity Behavior during the H-Mode in JFT-2M
- Ion Bernstein Wave Experiment on JFT-2M Tokamak
- Enhancement of Wall Fueling and Improvement in nτ of a Beam-Heated H-Mode Discharge by Electron Cyclotron Wave in the JFT-2M Tokamak
- Measurement of Peripheral Electron Temperature by Electron Cyclotron Emission during the H-Mode Transition in JFT-2M Tokamak
- AN EXPERIMENTAL STUDY OF THE WPW SYNDROME : RELATIONSHIP BETWEEN BODY SURFACE MAP AND PREEXCITATION AREA OF THE EPICARDIUM
- INHOMOGENEOUS SPREAD OF EXCITATION IN THE SPECIAL CONDUCTING SYSTEM
- RELATIONSHIP BETWEEN A SERIAL CHANGE OF THE TORSO SURFACE POTENTIAL DISTRIBUTION AND THE EPICARDIAL SEQUENCE OF VENTRICULAR ACTIVATION : PROCEEDINGS OF THE 38TH ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
- EXPERIMENTAL STUDY OF BILATERAL BUNDLE BRANCH BLOCK IN ISOLATED DOGS' HEART : PROCEEDINGS OF THE 38TH ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
- PJ-227 Evidence for Impaired Cardiac Autonomic Nerve Reflex during Balloon Coronary Occlusion in Patients with Diabetes(Autonomic nervous system-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Electronic States of the Antiferromagnet UGa_3 : Condensed Matter: Electronic Properties, etc.
- Magnetoresistance and de Haas-van Alpen Effect in UB_4
- Magnetic Phase Diagram in UCd_
- Electrical and Thermal Properties of UB_2
- de Haas-van Alpen Oscillations and Magnetic Properties of YbPb_3 and EuPb_3
- Study of Magnetic Excitation in Singlet-Ground-State Magnets CsFeCl_3 and RbFeCl_3 by Nuclear Magnetic Relaxation
- Softening Effect of Magnetic Excitation on Nuclear Spin-Lattice Relaxation of ^Cs in Singlet-Ground-State Magnet CeFeCl_3
- PJ-779 Predictive Value of Surrogate Markers of Effective Reperfusion on Greater Myocardial Salvage in Patients with Acute Myocardial Infarction(Myocardial ischemia-reperfusion, basic/clinical-6 (IHD) PJ136,Poster Session (Japanese),The 70th Anniversary A
- The Distal Protection During Primary Percutaneous Coronary Intervention Alleviates the Adverse Effects of Large Thrombus Burden on Myocardial Reperfusion
- Distal Protection during Primary Percutaneous Coronary Intervention Improves Myocardial Reperfusion of Infarct-Related Artery with High-Burden Thrombus Formation(Myocardial Ischemia-reperfusion, Clinical 2 (IHD), The 69th Annual Scientific Meeting of the
- OE-184 Delayed Stent Fracture and Restenosis after Successful Implantation of Sirolimus-Eluting Stent(Coronary revascularization, PCI-02, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-570 The Impact of Immediate Elastic Recoil on Late Restenosis After Balloon Angioplasty in Small Coronary Arteries(Restenosis after Angioplasty, Basic/Clinical 7 (IHD) : PJ95)(Poster Session (Japanese))
- OJ-115 The Long-Term Outcomes of Cutting Balloon Angioplasty With Provisional Stenting in Small Coronary Arteries : A Comparison With Primary Stenting(Coronary Revascularization, PICA/Stent/DCA/Rotablator/New Device 2 (IHD) : OJ14)(Oral Presentation (Japa
- Interplay between Magnetism and Superconductivity in URu_2Si_2 Studied by Neutron Scattering Experiments
- PJ-062 Can Further Vagal Modification be Achieved by a Superior Vena Cava Isolation Added to the Pulmonary Vein Isolation?(Arrhythmia, therapy-16, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-061 The Predictor of Long-Term Success in Patients with Early Recurrence of Atrial Fibrillation After Pulmonary Vein Isolation(Arrhythmia, therapy-16, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-066 Autonomic Nervous Remodeling Toward a Vagal Tonic State Could be Caused by the Perpetuation of Atrial Fibrillation(Arrhythmia, diagnosis/pathophysiology/EPS-01, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Electron Cyclotron Heating and Pre-Ionization in the JFT-2 Tokamak
- 胆道閉鎖症術後患児におけるMRIを用いた肝形態機能の経時的変化の検討
- Theoretical Investigation of Magnetic Structure and Hyperfine Field of NaCl-Type FeN
- Electronic,Structural and Magnetic Properties of Transition-Metal Mononitrides
- Edge Pedestal During the H-Mode of a Tokamak Plasma Measured by the Electron Cyclotron Emission
- Diamagnetic Measurement of JFT-2 Plasma Heated by Neutral Beam Injection
- Involvement of general transcriptional coactivator PC4 in the transcription of medaka fish intestine-specific membrane guanylyl cyclase gene (OlGC6).
- Enterotoxin/guanylin receptor type guanylyl cyclases in non-mammalian vertebrates
- Metal Impurity Reduction by Working Gas Injection in a Tokamak
- On the Origin of Arcing in a Tokamak
- Measurement of Surface Temperature of a Limiter and a Study on Plasma Confinement Characteristics
- Sequence Analysis of cDNA and Genomic DNA, and mRNA Expression of the Medaka Fish Homolog of Mammalian Guanylyl Cyclase C
- Molybdenum, Carbon and Silicon Carbide Limiter Experiment in the JFT-2 Tokamak
- Characterization of Two Types of Cytochrome P450 Aromatase in the Serial-sex Changing Gobiid Fish, Trimma okinawae
- EXPRESSION OF AD4BP/SF-1 IN RED-SPOTED DWARF GOBY, TRIMMA OKINAWAE, DURING MULTI-SEX CHANGE(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- TWO TYPES OF P450 AROMATASE DURING SERIAL ADULT SEX CHANGE IN GOBIID FISH Trimma okinawae : cDNA ISOLATION AND mRNA EXPRESSION
- Diverse forms of guanylyl cyclases in medaka fish -- their genomic structure and phylogenetic relationships to those in vertebrates and invertebrates.
- Extended String Order Parameter in the (S=1, 1/2) Mixed Spin Chain(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- The Trimer State of a (S=1,S=1/2) Ferrimagnetic Spin Chain as the Exact Ground State
- Magnetization Process of (S=3/2, S=1) Anisotropic Ferrimagnetic Spin Chain(Low Dimensional Systems, Field-Induced Phase Transitions and Dynamics in Quantum Spin Systems)
- Numerical Study of the Magnetization Process of Nanoscale Ferrimagnetic Ring Mn_6R_6(Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- Experimental Study on Limit of the Safety Factor in JFT-2 Tokamak
- Expression and Genomic Organization of A Medaka Fish Novel Membrane Form of Guanylyl Cyclase/Orphan Receptor
- Localization of the Nitric Oxide/cGMP Signaling Pathway-Related Genes and Influences of Morpholino Knock-Down of Soluble Guanylyl Cyclase on Medaka Fish Embryogenesis
- Expression of Membrane-Bound and Soluble Guanylyl Cyclase mRNAs in Embryonic and Adult Retina of the Medaka Fish Oryzias latipes
- Mass Spectrometric Analysis of Phosphoserine Residues Conserved in the Catalytic Domain of Membrane-Bound Guanylyl Cyclase from the Sea Urchin Spermatozoa
- A mRNA for Membrane Form of Guanylyl Cyclase Is Expressed Exclusively in the Testis of the Sea Urchin Hemicentrotus pulcherrimus
- Formation of Mature Egg Envelope Subunit Proteins from Their Precursors (Choriogenins) in the Fish, Oryzias latipes: Loss of Partial C-Terminal Sequences of the Choriogenins
- Analysis of chorion hardening of eggs of rainbow trout, Oncothynchus mykiss
- EXPRESSION OF PARTICULATE AND SOLUBLE GUANYLYL CYCLASE mRNA IN THE MEDAKA RETINA(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- INTRACELLULAR DAMAGE OF DNA INDUCED BY TRITIATED WATER AND ITS RESTORATION
- MRI Findings in the Liver in Biliary Atresia Patients after the Kasai Operation
- 3,4-Dihydroxyphenylalanine(DOPA) Decarboxylase Deficiency and Resultant High Levels of Plasma DOPA and Dopamine in Unfavorable Neuroblastoma
- Molecular Phylogenetic Analysis of Diverse Forms of Echinoderm Guanylyl Cyclases(Biochemistry)
- PJ-166 Can Additional RF Deliveries after Successful Pulmonary Vein Isolation Reduce the Recurrence of Atrial Fibrillation?(Arrhythmia, Non-Pharmacological Therapy 6 (A) : PJ28)(Poster Session (Japanese))
- PJ-162 Low Incidence of Recovery of Atrio-Venous Electrical Connection after Superior Vena Cava Isolation : Comparison with Pulmonary Vein Isolation(Arrhythmia, Non-Pharmacological Therapy 5 (A) : PJ27)(Poster Session (Japanese))
- PJ-160 Higher-Power RF Ablation with Guidance Using Basket Catheter Does not Increase Incidence of Pulmonary Vein (PV) Stenosis after PV Isolation.(Arrhythmia, Non-Pharmacological Therapy 5 (A) : PJ27)(Poster Session (Japanese))
- PJ-011 Left Atrial Macroreentrant Tachycardia can Mimic the Electrocardiographic Characteristics of Atrial Flutter after Linear Ablation of Cavo-Tricuspid Annulus(Arrhythmia, Diagnosis/Pathophysiology/EPS 4 (A): PJ2)(Poster Session (Japanese))
- THE INFLUENCE OF SINGLE OR REPEATED PHLEBOTOMY ON THE PHYSIOLOGICAL CONDITION OF NORMAL AND DISEASED RATS
- Expression of Sperm-Activating Peptide IV Receptor-Associated Membrane Guanylyl Cyclase in the Testis of the Sea Urchin Diadema setosum
- Expression of Sperm-Activating Peptide IV Receptor-Associated Membrane Guanylyl Cyclase in the Testis of the Sea Urchin Diadema setosum
- Primary structure of a 120kDa protein associated with the fucose sulfate glycoconjugate constituting the acrosome reaction-inducing substance of the sea urchin, Hemicentrotus pulcherrimus
- Electron Heat Conduction Coefficients at the Temperature Rise Phase and the Internal Disruption during the Sawtooth Oscillation of Tokamak
- Differential Expression of Two Cathepsin Es during Metamorphosis-Associated Remodeling of the Larval to Adult Type Epithelium in Xenopus Stomach
- DIFFERENTIAL EXPRESSION OF TWO CATHEPSIN Es IN XENOPUS LAEVIS FOREGUT DURING THE METAMORPHOSIS(Biochemistry)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- The Guanylyl Cyclase Family in Medaka Fish Oryzias latipes
- Expression and Exon/Intron Organization of Two Medaka Fish Homologs of the Mammalian Guanylyl Cyclase A^1
- Characterization of Sea Urchin Sperm Membrane Proteins which Interact with a Major Acrosome Reaction-Inducing Substance, Fucose Sulfate Glycoconjugate
- Esophageal Leads Can be Useful for Determining the Strategy of PV Ablation to Avoid Complications Associated with the Esophagus(Arrhythmia, Non-pharmacological Therapy 4 (A), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Circumferential Pulmonary Vein Isolation is More Effective for Curing Paroxysmal Atrial Fibrillation than Segmental Pulmonary Vein Ostial Isolation(Arrhythmia, Non-pharmacological Therapy 10 (A), The 69th Annual Scientific Meeting of the Japanese Circulat
- Circumferential PV Isolation Significantly Reduces Difference in AF Recurrence between Persistent and Paroxysmal AF as Compared to Segmental PV Isolation(Arrhythmia, Non-pharmacological Therapy 10 (A), The 69th Annual Scientific Meeting of the Japanese Ci
- Electrophysiological Pulmonary Vein Antrum Ablation with Multielectrode Basket-catheter is Effective for Curing AF : Efficacy of Minimally Extensive Pulmonary Vein Isolation(Arrhythmia, Non-pharmacological Therapy 5 (A), The 69th Annual Scientific Meeting
- FRS-032 How Does Triggered Ectopic Activity Induce Atrial Fibrillation within the Pulmonary Veins?(Arrhythmia-Clinical : Advances in Diagnosis and Management (A) : FRS5)(Featured Research Session (English))
- Identification of tandem organization of soluble guanylyl cyclase α_1 and β_1 subunit genes in the Japanese pufferfish (Fugu rubripes) genome: comparison with their human homologues
- Inhomogeneous Spread of Excitation in Canine Purkinje Fibers
- Genomic Structure and Expression of the Sea Urchin Soluble Guanylyl Cyclase β Subunit Gene
- Dephosphorylation of Autophosphorylated Regulatory Subunit of Sea Urchin Sperm cAMP-Dependent Histone Kinase by an Endogenous Protein Phosphatase