ラットSKD3 cDNAのクローニングと精巣におけるSKD3の発現
スポンサーリンク
概要
- 論文の詳細を見る
To analyze the function of heat shock protein (HSP) in spermatogenesis, rat suppressor of potassium transport defect 3 (SKD3) cDNA, a novel member of the HSP family, was cloned in rat testis, and its expression and localization was investigated in rat testis at the mRNA and protein levels. The cDNA and predicted amino acid sequence of rat SKD3 showed approximately 95.6% and 97.0% homology, respectively, with mouse SKD3. All motifs recognized in mouse were conserved in rat. SKD3 mRNA (2.3 kbs) was restricted to the testis among the tissues investigated. SKD3 mRNA was detected by in situ hybridization in spermatogonia, spermatocytes, Leydig cells, and Sertoli cells. Immunoreactive SKD3 from whole rat testis and a Leydig cell line (LC540) was seen at 76 kDa by Western blotting. SKD3 immunoreactive cells were Leydig and Sertoli cells. Although the cellular localizations of SKD3 mRNA and protein were somewhat inconsistent, rat SKD3 cDNA was characterized and various expression patterns of SKD3 mRNA and protein in rat testis were revealed in the present study.
- 日本繁殖生物学会の論文
著者
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Kitamura Nobuo
Department of Cardiovascular Surgery, Kyoto Prefectural University of Medicine
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HONDO Eiichi
Department of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University
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KISO Yasuo
Department of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University
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NAGAHAMA Yoshitaka
Laboratory of Reproductive Biology, National Institute for Basic Biology
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本道 栄一
Department of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University
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Yamada Junzo
Department Of Veterinary Anatomy Obihiro University Of Agriculture And Veterinary Medicine
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Kiso Yasuo
Department Of Veterinary Anatomy Faculty Of Agriculture Yamaguchi University
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Manabe Noboru
Unit Of Anatomy And Cell Biology Department Of Animal Sciences Kyoto University
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Yamada Junzo
Department Of Veterinary Anatomy Faculty Of Veterinary Medicine Hokkaido University
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小林 亨
Laboratory of Reproductive Biology, National Institute of Basic Biology
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会田 恒彦
Department of Veterinary Anatomy, Obihiro University of Agriculture & Veterinary Medicine
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NAMBA Yasuharu
Department of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University
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会田 恒彦
帯広畜産大学・畜産学部・家畜解剖学講座
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Kobayashi Tohru
Laboratory of Reproductive Biology, National Institute for Basic Biology
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Kitamura Nobuo
Department Of Cardiovascular And Thoracic Surgery Kyoto Prefectural University Of Medicine
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Nagahama Yoshitaka
Laboratory Of Reproduction National Institute For Basic Biology
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AITA Tsunehiko
Department of Veterinary Anatomy, Obihiro University of Agriculture & Veterinary Medicine
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小林 亨
Laboratory Of Reproductive Biology National Institute For Basic Biology
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Kobayashi Tohru
Laboratory Of Reproduction National Institute For Basic Biology
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MANABE Noboru
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
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NAGAHAMA Yoshitaka
Laboratory of Reproductive Biology, National Institute of Basic Biology
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YAMADA Junzo
Department of Veterinary Anatomy, Obihiro University of Agriculture & Veterinary Medicine
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AITA Tsunehiko
Department of Veterinary Anatomy, Obihiro University of Agriculture & Veterinary Medicine
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