Isolation of Granulated Metrial Gland Cells, a Uterine Form of Natural Killer Cell, by Magnetic Immunobeads/Particles
スポンサーリンク
概要
- 論文の詳細を見る
Significant numbers of cells known by the name granulated metrial gland (GMG) cells, or uterine natural killer (NK) cells, are present in the murine uterus only during pregnancy. The functions of GMG cells remain incompletely defined, largely due to technical difficulties in isolating and culturing these cells. New methods for purifying large numbers of GMG cells are required. This study examined two methods for isolating GMG cells from the murine pregnant uterus based upon magnetic immunobeads and immunoparticles. By either approach, the yield of GMG cells was 1 × 104 cells/metrial gland. Isolations using immunobeads gave cell suspensions with 82% in purity and 70% in viability. Isolations using immunoparticles gave cell suspensions with 62% in purity and 67% in viability. GMG cells isolated by the magnetic method retained an ultrastructure similar to in vivo GMG cells. These results establish that immunomagnetic approaches are useful for isolation of GMG cells from the pregnant murine uterus.
- 日本繁殖生物学会の論文
著者
-
OKADA Toshiya
Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of
-
KUSAKABE Ken
Department of Integrated Structural Bioscience, Division of Veterinary Science, Graduate School of L
-
KISO Yasuo
Department of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University
-
Sasaki Fumihiko
Department of Internal Medicine, Fukui Cardiovascular Center
-
Kusakabe K
Department Of Laboratory Animal Science Division Of Veterinary Science Graduate School Of Life And E
-
MORIKAWA Yoshio
Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of
-
TOKUNAGA Yoshiko
Department of Veterinary Anatomy, College of Agriculture, Osaka Prefecture University, 1-1 Gakuen-ch
関連論文
- Novel Cataract Mouse Model Using ddY Strain: Hereditary and Histological Characteristics
- Spontaneous Endometrial Hyperplasia in the Uteri of IL-2 Receptor Beta-Chain Transgenic Mice
- Effects of low protein intake on the development of the remaining kidney in subtotally nephrectomized immature rats : expression of inducible and endothelial NO synthase
- Phagocytosis mechanism of apoptotic granulosa cells regulated by milk-fat globule-EGF factor 8
- The relationship between apoptosis and splenocyte depletion in rats following ethanol treatment
- -79- BRONCHIAL HYPERRESPONSIVENESS IN PATIENTS WITH CHRONIC LEFT HEART FAILURE : Congestive Heart Failure : FREE COMMUNICATIONS(I) : PROCEEDINGS OF THE 51th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- -237-PROLONGED IMPROVEMENT OF HEMODYNAMICS AFTER A SINGLE ADMINISTRATION OF ORAL DOBUTAMINE
- IGF-I過剰発現はマウス胎盤形成期に流産を引き起こす
- ラットSKD3 cDNAのクローニングと精巣におけるSKD3の発現
- 子宮NK細胞はマウス妊娠子宮においてEGFを産生する
- Starvation induces the formation of giant mitochondria in gastric parietal cells of guinea pigs
- Histamine Increased the Uptake of Rhodamine 123 in Mitochondria of Living Parietal Cells in Cultured Gastric Glands from Starved Guinea Pigs
- Histology of Vermiform Appendix-Like Organ in Slow Loris
- Histological Study on Intestinal Diverticulum of Tree Shrew (Tupaia Javanica)
- Effects of maternal uninephrectomy on the development of fetal rat kidney : apoptosis and the expression of oncogenes
- Perinatal development of the rat kidney : Apoptosis and epidermal growth factor
- Effects of maternal uninephrectomy on the development of fetal rat kidney with special reference to the proliferative activity and epidermal growth factor (EGF).
- Uterine Natural Killer Cells in Perforin and β_2-Microglobulin Deficient Mice(Anatomy)
- Effect of Danazol on NK Cells and Cytokines in the Mouse Uterus
- Effect of Danazol on NK Cells and Cytokines in the Mouse Uterus
- Histological Study of Granulated Metrial Gland (GMG) Cells in Beige (DA-bg/bg) Rats
- Localization of Interleukin-6 Receptor mRNA in the Pregnant and Non-pregnant Mouse Uterus
- Differentiation and Elimination of Uterine Natural Killer Cells in Delayed Implantation and Parturition Mice
- Ultrastructural Study of Uterine Natural Killer Cells Found in Pregnant, Interleukin-2 Receptor β-chain Overexpressed Transgenic Mice
- Involvement of the Fas Ligand and Fas System in Apoptosis Induction of Mouse Uterine Natural Killer Cells
- Isolation of Granulated Metrial Gland Cells, a Uterine Form of Natural Killer Cell, by Magnetic Immunobeads/Particles
- Compensatory Renal Growth in Uninephrectomized Immature Rats: Proliferative Activity and Epidermal Growth Factor
- Effects of Bisphenol A (BPA) on Placentation and Survival of the Neonates in Mice
- Apoptosis of Rat Uterine NK Cells In Vitro
- Agrin Pathway is Controlled by Leukemia Inhibitory Factor (LIF) in Murine Implantation
- Upregulation of Indian Hedgehog Gene in the Uterine Epithelium by Leukemia Inhibitory Factor During Mouse Implantation
- IGF-I過剰発現はマウス胎盤形成期に流産を引き起こす
- Age-related changes in growth hormone-immunoreactive cells in the anterior pituitary gland of Jcl : Wistar-TgN (ARGHGEN) 1Nts rats (Mini rats)
- Isolation of Granulated Metrial Gland Cells, a Uterine Form of Natural Killer Cell, by Magnetic Immunobeads/Particles
- Histological Observation of the Proper Gastric Gland in Minke Whale, Balaenoptera acutorostrata.