Effect of Danazol on NK Cells and Cytokines in the Mouse Uterus
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概要
- 論文の詳細を見る
Danazol, which has been used as a medicine for endometriosis, has a valid effect in pretreatment of patients receiving in vitro fertilization and embryo transfer, although its reproductive mechanism remains unclear. BALB/c mice were subcutaneously injected with danazol for 2 weeks. Blood and uteri were collected and cytokines were assayed. Following danazol treatment, an increase in pregnancy ratio was evident that was accompanied by up-regulation in serum macrophage-colony stimulating factor (M-CSF). RT-PCR analysis revealed that expression of M-CSF and Ly49, a phenotypic marker of natural killer (NK) cells, was up-regulated in the uteri of the danazol-treated mice. In immunohistochemical analysis, M-CSF and Ly49, together with α5 integrin, were clearly detected in the endometrium of the danazol-treated mice with very similar pattern of localization. These results suggest that danazol has an effect to promote pregnancy that induces recruitment of NK cells and a concomitant increase in the expression of M-CSF and α5 integrin in the uterus.
- 日本繁殖生物学会の論文
著者
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KUSAKABE Ken
Department of Integrated Structural Bioscience, Division of Veterinary Science, Graduate School of L
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OTSUKI Yoshinori
Department of Anatomy and Cell Biology, Division of Life Sciences, Osaka Medical College
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Kusakabe K
Department Of Laboratory Animal Science Division Of Veterinary Science Graduate School Of Life And E
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Kusakabe Ken
Department Of Anatomy And Biology Osaka Medical College
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Nakamuta Nobuaki
First Department Of Oral Anatomy Kyushu Dental College
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Li Zhong-lian
Department Of Anatomy And Cell Biology Osaka Medical College
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Otsuki Yoshinori
Department Of Anatomy & Biology Osaka Medical College
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MORISHIMA Shoko
Department of Anatomy and Cell Biology, Division of Basic Medicine 1, Osaka Medical College
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Li Zhong-lian
Department Of Anatomy And Cell Biology Division Of Basic Medicine 1 Osaka Medical College
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Kokubu Keiji
The United Graduate School Of Veterinary Science Yamaguchi University
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Morishima Shoko
Department Of Anatomy And Cell Biology Osaka Medical College
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