Cloning of cDNA Encoding Thimet Oligopeptidase from Xenopus Oocytes and Regulation of the mRNA During Oogenesis(Molecular Biology)
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概要
- 論文の詳細を見る
We have isolated a cDNA clone for a zinc-requiring metallopeptidase in Xenopus oocytes from Xenopus ovary library using oligonucleotides synthesized on the basis of the partial amino acid sequence. The full-length 2,055 bp cDNA encodes a protein of 685 amino acid residues with a predicted molecular mass of 78,136 Da. The deduced amino acid sequence of this protein exhibits high similarity to that of human (74.1%), pig (75.3%) and rat (74.1%) thimet Oligopeptidase (TOP) [EC 3.4.24.15]. Expression of the cDNA in bacterial cells resulted in the production of an active metalloenzyme. Thus, we concluded that the metallopeptidase purified from Xenopus oocytes is a member of the TOP family. In Northern blot analyses, one major species of Xenopus-TOP (X-TOP) mRNA of 3.0 kb was expressed relatively strongly from early stage (III) of Xenopus oogenesis, its level decreasing in later stages (V and VI). This result suggests that the expression of X-TOP mRNA is regulated during Xenopus oogenesis.
- 社団法人日本動物学会の論文
- 2000-05-20
著者
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NAGAHAMA Yoshitaka
Laboratory of Reproductive Biology, National Institute for Basic Biology
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Ohe Yoshihide
Biosignal Research Center Institute For Molecular And Cellular Regulation Gunma University
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Miyamoto Kaoru
Biosignal Research Center Institute For Molecular And Cellular Regulation Gunma University
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Tokumoto M
Marine Science Institute University Of Texas At Austin
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ISHIKAWA Katsutoshi
Department of Biology, Faculty of Science, Shizuoka University
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TOKUMOTO Toshinobu
Department of Biology, Faculty of Science, Shizuoka University
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Okida Noriyuki
Department of Biology and Geosciences, Faculty of Science, Shizuoka University
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Tokumoto Mika
Laboratory of Reproductive Biology, National Institute for Basic Biology
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Tokumoto T
Department Of Biology And Geosciences Faculty Of Science National University Corporation Shizuoka Un
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Tokumoto Mika
Marine Science Institute University Of Texas At Austin
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Nagahama Y
Laboratory Of Reproductive Biology National Institute For Basic Biology
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Ishikawa K
Department Of Biology And Geosciences Faculty Of Science National University Corporation Shizuoka Un
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Nagahama Yoshitaka
Laboratory Of Reproduction National Institute For Basic Biology
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Ishikawa Katsutoshi
Department Of Biology And Geosciences Faculty Of Science Shizuoka University
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Tokumoto Toshinobu
Department Of Biology And Geosciences Faculty Of Science National University Corporation Shizuoka Un
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Tokumoto M.
Department Of Biology And Geosciences Faculty Of Science Shizuoka University : Japan Science And Tec
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