Immunological Detection and Characterization of Poly(A) Polymerase, Poly(A)-Binding Protein and Cytoplasmic Polyadenylation Element-Binding Protein in Goldfish and Xenopus Oocytes
スポンサーリンク
概要
- 論文の詳細を見る
Cytoplasmic polyadenylation regulates translational activation of dormant cyclin B1 mRNA stored in immature oocytes, a process required for the initiation of oocyte maturation in goldfish and Xenopus. As a first step towards understanding the molecular mechanisms of translational activation of cyclin B1 during oocyte maturation, we have isolated cDNA clones encoding proteins involved in cytoplasmic polyadenylation and produced specific antibodies against recombinant proteins. These include poly(A) polymerase (PAP), poly(A)-binding protein (PABP) and cytoplasmic polyadenylation element-binding protein (CPEB). Monoclonal antibodies raised against goldfish PAP recognized several forms of PAP in goldfish and Xenopus oocytes. Besides ordinary PAPs with high molecular weight (ca. 100 kDa), the antibodies also detected those with low molecular weight (ca. 40 kDa) that are present specifically in the cytoplasm, raising new players that might be responsible for cytoplasmic polyadenylation. An antibody against goldfish PABP showed for the first time in Xenopus oocytes the protein expression of PABPII, another PABP distinct from the well-characterized PABPI. Monoclonal antibodies raised against Xenopus CPEB recognized both unphosphorylated 62-kDa and phosphorylated 64-kDa forms but did not cross-react with goldfish CPEB, which was specifically detected by anti-goldfish CPEB monoclonal antibodies produced previously. The cDNAs, recombinant proteins and antibodies produced in this study are expected to provide useful tools for investigating the regulatory mechanisms of cyclin B1 translation during oocyte maturation in goldfish and Xenopus.
- 社団法人日本動物学会の論文
- 2001-04-10
著者
-
YAMASHITA Masamichi
Institute of Space and Astronautical Science (ISAS), Japan Aerospace Exploration Agency (JAXA)
-
NAGAHAMA Yoshitaka
Laboratory of Reproductive Biology, National Institute for Basic Biology
-
Mita Koichi
Laboratory Of Molecular And Cellular Interactions Division Of Biological Sciences Graduate School Of
-
Mita Koichi
Laboratory Of Molecular And Cellular Interactions Division Of Biologidal Sciences Graduate School Of
-
Yamashita Masakane
Laboratory of Molecular and Cellular Interactions, Graduate School of Science, Hokkaido University
-
Yamashita Masakane
Laboratory Of Molecular And Cellular Interactions Division Of Bioloaical Sciences Graduate School Of
-
Yamashita Masamichi
Institute Of Space And Astronautical Science
-
Yamashita Masamichi
Institute Of Space And Astronautical Science (isas) Japan Aerospace Exploration Agency (jaxa)
-
Nagahama Y
Laboratory Of Reproductive Biology National Institute For Basic Biology
-
Katsu Y
Okazaki National Res. Inst. Okazaki Jpn
-
Nagahama Yoshitaka
Laboratory Of Reproduction National Institute For Basic Biology
-
Yasujima Minoru
Dep. Of Lab. Medicine Hirosaki Univ. Graduate School Of Medicine
-
Nakahata Shingo
Laboratory of Molecular and Cellular Interactions, Division of Biological Sciences, Graduate School
-
Katsu Yoshinao
Laboratory of Reproductive Biology, National Institute for Basic Biology
-
Nakahata S
Laboratory Of Molecular And Cellular Interactions Division Of Biological Sciences Graduate School Of
関連論文
- Expression levels of hormone receptors and bone morphogenic protein in fins of medaka
- Effects of Strong Static Magnetic Fields on Amphibian Development and Gene Expression
- EFFECT OF A HIGH MAGNETIC FIELD ON AMPHIBIANS(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Tissue-specific Expression of Rhamnose-binding Lectins in the Steelhead Trout (Oncorhynchus mykiss)(Biochemistry & Molecular Biology)
- Japanese Red-Bellied Newts in Space - AstroNewt Experiment on Space Shuttle IML - 2 and Space Flyer Unit
- Growth of terrestrial cyanobacterium, Nostoc sp., on Martian Regolith Simulant and its vacuum tolerance
- The effect of hypergravity on bipolar spindle formation of meiosis II in Xenopus oocytes
- Effects of Hypergravity on Pituitary-Target Organs in the Frog, Xenopus laevis
- Promoter Activity and Chromosomal Location of the Rana rugosa P450 Aromatase (CYP19) Gene(Reproductive Biology)
- Morphagensis of Rice and Arabidopsis Seedlings in Space
- Morphogenesis of Rice and Arabidopsis Seedlings in Space
- Microorganisms and Plant of Autonomous Biological Systems (ABS) Samples
- Concluding Remarks of Autonomous Biologica Systems (ABS) Experiments
- Concluding Remarks of Autonomous Biological Systems (ABS) Experiments
- Performance of a Digital Video Camcorder for the Autonomous Biological System Experiment onboard Space Station Mir
- Microorganisms and Plant of Autonomous Biological Systems (ABS) Samples
- Analysis of Water in Autonomous Biological Systems (ABS) Samples
- GUT MOTILITY OF RANA TAGOI TAGOI TADPOLES OBSERVED THROUGH TRANSPARENT ABDOMINAL WALL(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- GONADOTROPIN RECEPTORS OF TWO TELEOST FISHES, AMAGO SALMON (ONCORHYNCHUS RHOSURUS) AND TILAPIA (OREOCHROMIS NILOTICUS) : cDNA CLONING AND EXPRESSION DURING OOCYTE DEVELOPMENT
- Autotropism under Microgravity Conditions Simulated by a Three-Dimensional Clinostat
- Cellular basis for the automorphic curvature of rice coleoptiles on a three-dimensional clinostat : possible involvement of reorientation of cortical microtubules
- MOLECULAR CLONING OF GLYCOPROTEIN HORMONE RECEPTOR FAMILY FROM MEDAKA OVARY
- Hydrotropic Response and Expression Pattern of Auxin-Inducible Gene, CS-IAA1, in the primary Roots of Clinorotated Cucumber Seedlings
- Formation and Stability of Complex Organic Compounds in Space Environments
- Abiotic Formation of Bioorganic Compounds in Space -Preliminary Experiments on Ground and Future Exobiology Experiments in Space-
- ラットSKD3 cDNAのクローニングと精巣におけるSKD3の発現
- ラット精巣において、プロラクチンはプロタミン2mRNAの発現を誘導する
- IN VITRO SPERMATOGENESIS IN FISH OVARY(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Dax1 REGULATES EXPRESSION OF P450arom IN MEDAKA FOLLICLES(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- CLONING AND ANALYSIS OF GONADAL EXPRESSION OF TESTICULAR TYPE SOX9 IN THE MEDAKA, ORYZIAS LATIPES(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- ORIGIN OF DMY, A SEX-DETERMINING GENE OF THE MEDAKA, ORYZIAS LATIPES(Genetics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- DMY INDUCE MALE DEVELOPMENT IN XX TRANSGENIC MEDAKA FISH(Endocrinology &Genetics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Oryzias curvinotus Has DMY, a Gene That Is Required for Male Development in the Medaka, O. latipes(Developmental Biology)
- A STRAIN HAVING A ZZ/ZW SEX DETERMINATION SYSTEM DERIVED FROM A NATURALLY OCCURRING RECESSIVE MUTATION IN THE MEDAKA, ORYZIAS LATIPES(Genetics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- MUTATIONS AFFECTING SEX DETERMINATION OR DIFFERENTIATION FROM WILD POPULATIONS OF THE MEDAKA, ORYZIAS LATIPES(Genetics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- EXPRESSION OF DMY IN MEDAKA GONAD DURING EARLY TESTICULAR DEVELOPMENT(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Construction of BAC libraries derived from the ascidian Ciona intestinalis
- Construction of a BAC library derived from the inbred Hd-rR strain of the teleost fish, Oryzias latipes
- STEREOSCOPIC IMAGES IN THE SMOOTH MUSCLE FIBERS OF THE SMALL AND LARGE INTESTINES IN TADPOLES (RHACOPHORUS OWSTONl) DURING METAMORPHOSIS(Cell Biology and Morphology & Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological
- COMPARATIVE HISTOLOGICAL STUDIES OF THE LIVERS IN AMPHIBIANS ASSOCIATED WITH THEIR DIVERGED MODE OF LIVING(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- A Spaceflight Experiment for the Study of Gravimorphogenesis and Hydrotroposm in Cucumber Seedlings
- Space Agriculture Concept on Mars and Model Plant Species
- EFFECTS OF HIGH GRAVITY ON AMPHIBIAN FERTILIZED EGGS AND EMBRYOS(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- EFFECTS OF GRAVITY DURING AMPHIBIAN METAMORPHOSIS(Developmental Biology & Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- cDNA cloning of a stage-specific gene expressed during HCG-induced spermatogenesis in the Japanese eel
- Hormonal induction of all stages of spermatogenesis in germ-somatic cell coculture from immature Japanese eel testis
- Acquisition of Potential for Sperm Motility in vitro in Japanese Eel Anguilla japonica
- Human Recombinant Activin Induces Proliferation of Spermatogonia in vitro in the Japanese Eel Anguilla japonica
- Gonadal structure and P450scc and 3β-HSD immunoreactivity in the gobiid fish Trimma okinawae during bidirectional sex change
- EXPRESSION OF GONADOTROPIN RECEPTORS IN THE SERIAL SEX CHANGING GOBY, TRIMMA OKINAWAE(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Characterization of Two Types of Cytochrome P450 Aromatase in the Serial-sex Changing Gobiid Fish, Trimma okinawae
- EXPRESSION OF AD4BP/SF-1 IN RED-SPOTED DWARF GOBY, TRIMMA OKINAWAE, DURING MULTI-SEX CHANGE(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- MOLECULAR INTERACTION BETWEEN PROGESTOGEN AND ANDROGENS VIA PROGESTOGEN RECEPTOR IN JAPANESE EEL (ANGUILLA JAPONICA)(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- DYNAMICS OF CELL CYCLE RELATED GENES AFTER HORMONAL INDUCTION OF SPERMATOGENESIS IN THE JAPANESE EEL, ANGUILLA JAPONICA(Cell Biology and Morphology & Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Socie
- UP- AND DOWN-REGULATED GENES AFTER THE INDUCTION OF XY SEX-REVERSAL BY ESTROGENS IN A TELEOST, TILAPIA, OREOCHROMIS NILOTICUS(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- TWO TYPES OF P450 AROMATASE DURING SERIAL ADULT SEX CHANGE IN GOBIID FISH Trimma okinawae : cDNA ISOLATION AND mRNA EXPRESSION
- COMPARISON OF THE LIGAND SPECIFICITY OF PROGESTOGEN RECEPTORS α AND β
- DIFFERENTIATION OF GERM CELL-SUPPORTING CELLS INTO SERTOLI CELLS DURING TESTICULAR DIFFERENTIATION IN A TELEOST, TILAPIA(Developmental Biology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- DYNAMICS OF CELL CYCLE RELATED GENES AFTER HORMONAL INDUCTION OF SPERMATOGENESIS IN THE MALE JAPANESE EEL, ANGUILLA JAPONICA(Developmental Biology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- The vasa-like gene, olvas, identifies the migration path of primordial germ cells during embryonic body formation stage in the medaka, Oryzias latipes
- PCNA Protein Expression during Spermatogenesis of the Japanese Eel (Anguilla japonica)
- MODIFICATIONS TO PROTEASOMAL SUBUNITS DURING OOCYTE MATURATION IN GOLDFISH(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- CLONING OF cDNA ENCODING SUBTYPES OF MEMBRANE PROGESTIN RECEPTOR (mPR) FROM GOLDFISH OVARY AND EXPRESSION ANALYSIS OF CLONED GENE(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Induction and inhibition of oocyte maturation by EDCs in zebrafish
- PHOSPHORYLATION OF α4 SUBUNIT OF THE 26S PROTEASOME BY CASEIN KINASE Iα DURING MEIOSIS IN GOLDFISH OOCYTES(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- CLONING OF cDNA ENCODING MEMBRANE PROGESTIN RECEPTOR α (mPRα) FROM GOLDFISH OVARY AND EXPRESSION ANALYSIS OF CLONED GENE(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- IDENTIFICATION OF α-SUBUNITS OF XENOPUS 20S PROTEASOME AND ANALYSIS OF CHANGES DURING THE MEIOTIC CELL CYCLE(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- INDUCTION AND INHIBITION OF OOCYTE MATURATION BY EDCs IN ZEBRAFISH(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Identification of -type subunits of the Xenopus 20S proteasome and analysis of their changes during the meiotic cell cycle
- IDENTIFICATION OF THREE XENOPUS PROTEASOME α-SUBUNITS AND ANALYSIS OF MODIFICATIONS DURING MEIOTIC CELL CYCLE(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- IDENTIFICATION OF A PROTEIN KINASE WHICH PHOSPHORYLATES α4 SUBUNIT OF THE 26S PROTEASOME IN GOLDFISH OOCYTES(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- DIETHYLSTILBESTROL INDUCES FISH OOCYTE MATURATION(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- EFFECTS OF ABNORMAL ENVIRONMENTS ON AMPHIBIAN DEVELOPMENT(Behavior Biology and Ecology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Comparison of antioxidant defense system in diploid and haploid anurans(Biochemistry)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- THE EFFECTS OF REACTIVE OXYGEN SPECIES ON AMPHIBIAN AGING(Biochemistry)(Proceedings of the Seventieth Annual Meeting of the Zoological Society of Japan)
- Amphibian Amplexus in Microgravity
- HIGH GRAVITY INHIBITS EMBRYONIC DEVELOPMENT OF AMPHIBIANS(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Pistil and stamen of lily flowers bend upward by light
- Response of allelochemicals under pseudo-microgravity in sunflower plant (Helianthus annuus L. cv. Taiyo)
- Distribution of L-DOPA in the root of velvet bean plant (Mucuna pruriens L.) and gravity
- Fate of allelopathic substances in space - Allelopathy of velvet bean plant and gravity
- Life History of Amphibians and Gravity
- The Frog in Space (FRIS) Experiment Onboard Space Station Mir: Final Report and Follow-on Studies
- The Frog in Space(FRIS)Experiment Onboard Space Station Mir:Final Report and Follow-on Studies (特集:日本の宇宙生物学実験)
- BIO EXPERIMENT ON SPACE FLYER UNIT : EFFECTS OF GRAVITY ON EARLY DEVELOPMENT OF JAPANESE RED BELLIED NEWT
- VARIATION IN THE RESPONSE TIME FOR THE STARTLE REACTION OF ANURAN TADPOLES(Behavior Biology and Ecology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- THE STARTLE RESPONSE OF ANURAN TADPOLES AND ITS CHANGES WITH GROWTH(Physiology)(Proceedings of the Seventieth Annual Meeting of the Zoological Society of Japan)
- VIP- AND NO-NERVE FIBRES IN THE NERVE PLEXUS OF THE ALIMENTARY TRACT OF Xenopus laevis TADPOLES(Physiology)(Proceedings of the Seventieth Annual Meeting of the Zoological Society of Japan)
- Behavioral responses of six amphibians and one fish to the parabolic flight stimulation(Physiology)(Proceedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan)
- XENOPUS PUMILIO HAS A ROLE IN TRANSLATIONAL REPRESSION OF CYCLIN B1 mRNA(Developmental Biology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Immunological Detection and Characterization of Poly(A) Polymerase, Poly(A)-Binding Protein and Cytoplasmic Polyadenylation Element-Binding Protein in Goldfish and Xenopus Oocytes
- Volatile allelopathy in velvet bean (Mucuna pruiens L.) and gravity
- Reduced allelopathic inhibition of lettuce (Lactuca sativa) growth caused by velvet bean (Mucuna pruriens) under 3D-clinorotation
- ENTOMOPHAGY AS PART OF A SPACE DIET FOR HABITATION ON MARS
- Morphogenesis and cell wall changes in maize shoots under simulated microgravity conditions
- Changes in Plant Growth Processes under Microgravity Conditions Simulated by a Three - Dimensional Clinostat
- Organization of Cytoplasmic Microtubules during Maturation of Goldfish Oocytes
- Structural changes in the smooth muscle fibers of digestive tracts in tadpoles(Rhacophorus owstoni) during metamorphosis(Cell Biology and Morphology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- HISTOCHEMICAL CHANGE WITH METAMORPHOSIS IN THE SMOOTH MUSCLES OF THE ALIMENTARY CANAL IN TADPOLES(Rhacophorus owstoni)(Developmental Biology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- Startle Response and Turning Bias in Microhyla Tadpoles(Behavior Biology)