Production of the Oocyte Maturation-Inducing Substance of Starfish by Heat Treatment of S-Adenosylmethionine
スポンサーリンク
概要
- 論文の詳細を見る
1-Methyladenine(1-MeA)has been identified as the oocyte maturation-inducing substance(MIS)in starfish, but little is known about its biosynthesis. This study showed that starfish MIS activity was present in a reactant derived from S-adenosylmethionine(SAM)by heat treatment. In vitro MIS production was markedly dependent on the temperature of the SAM solution : it increased as the temperature was raised, and reached a plateau within 5 min upon boiling, although hardly only MIS was observed upon incubation below 20℃. MIS production was also dependent on the solution pH. Analyses by high-performance liquid chromatography and thin-layer chromatography showed that the MIS was 1-MeA, though the maximum amount of 1-MeA obtained from SAM by boiling was only 0.3% of the initial SAM amount. Further-more, use of S-[^<14>C-methyl]SAM showed that a methyl group of 1-MeA was transferred from the SAM. Thus, it is possible that 1-MeA may be produced from SAM in vivo.
- 社団法人日本動物学会の論文
著者
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SANEYOSHI MINEO
Department of Biological Sciences, Teikyo University of Sciences and Technology
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NAGAHAMA Yoshitaka
Laboratory of Reproductive Biology, National Institute for Basic Biology
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YOSHIKUNI Michiyasu
Laboratory of Repriductive Biology, National Institute for Basic Biology
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Yoshikuni Michiyasu
National Institute for Basic Biology
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Saneyoshi Mineo
Department Of Biological Sciences Teikyo University Of Sciences And Technology
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Saneyoshi Mineo
Department Of Biological Science Teikyo University Of Science And Technology
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Saneyoshi Mineo
Dept. Of Biol. Sci. Teikyo Univ. Sci. & Tech.
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Yasumasu Ikuo
Department of Biology, School of Education, Waseda University
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Yoshikuni Michiyasu
Laboratory Of Repriductive Biology National Institute For Basic Biology
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Yoshikuni M
Laboratory Of Reproductive Biology National Institute For Basic Biology
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Mita Masatoshi
Department of Biosciences, School of Science and Engineering, Teikyo University
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Yasumasu I
Department Of Biology School Of Education Waseda University
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Yasumasu Ikuo
Department Of Biology Colege Of General Education University Of Tokyo
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Mita Masatoshi
Department Of Applied Physics Faculty Of Engineering Osaka City University
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Nagahama Yoshitaka
Laboratory Of Reproduction National Institute For Basic Biology
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