Purification and Characterization of Vanillyl-Alcohol Oxidase from Byssochlamys fulva V107

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概要

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• Vanilly-alcohol oxidase from Byssochlamys fulva V107 was purified to apparent homogeneity as shown by SDS-PAGE and gel-permeation HPLC. The enzyme is a homodimeric flavoenzyme consisting of two 58 kDa subunits. It catalyzes the dehydrogenation of different 4-hydroxybenzylic structures, including the conversion of 4-hydroxybenzylalcohols such as vanillyl alcohol to the corresponding aldehydes, eugenol to coniferyl alcohol, and 4-alkylphenols to 1-(4-hydroxyphenyl)alcohols. The latter reaction was S-stereospecific and was used for the synthesis of S-1-(4-hydroxyphenyl)ethanol and -propanol with enantiomeric excesses of 81.9 and 86.0%, respectively. The catalytic and structural similarities to a Penicillium vanillyl-alcohol oxidase and Pseudomonas 4-alkylphenol methylhydroxylases are discussed.

• 社団法人日本生物工学会の論文
• 1999-03-25

著者

• Nagasawa Toru

  Department of Biomolecular Science, Gifu University

• 森田 浩

  大阪大学 大学院 情報科学研究科

• 長澤 透

  岐阜大・工・生命工

• Sugio Tsuyoshi

  Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an

• Nagasawa T

  Department Of Biomolecular Science Gifu University

• Sugio Tsuyoshi

  Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers

• Sugio Tsuyoshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University

• Sugio Tsuyoshi

  Department Of Biological Function And Genetic Resources Science Okayama University

• FURUKAWA HIROTAKA

  Department of Biological Function and Genetic Resources Science, Okayama University

• MARCO WIESER

  Department of Biomolecular Science, Faculty of Engineering, Gifu University

• MORITA HIROSHI

  Chisso Corporation, Yokohama Research Center

• Nagasawa Toru

  Department Of Agricultural Chemistry Kyoto University

• Wieser Marco

  Department Of Biomolecular Science Faculty Of Engineering Gifu University

• Furukawa Hirotaka

  Department Of Applied Physics And Chemistry University Of Electro-communications

• Sugio T

  Okayama Univ. Okayama Jpn

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