Distribution of Hydantoin Hydrolyzing Activity in Microorganisms : Microbial Transformation of Hydantoins to Amino Acids(I)

概要

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The distribution of hydantoin hydrolyzing activity in microorganisms was investigated and high activity was found in various bacteria belonging to Pseudomonas, Aerobacter, Corynebacterium, Mycobacterium and in actinomycetes. Hydrolysis of DL-5-(2-methylthioethyl)hydantoin was carried out with the microorganisims and the product was identified as N-carbamyl-D-methionine by physicochemical methods. The culture conditions were studies with a strain of Pseudomonas striata and cells containg high activity were obtained when they grew at 30℃ for 12 hr in a medium containgin 20 g meat extract, 6 g glycerol, 1 g hydantoin, 2g KH_2PO_4 1 g MgSO_4・7H_2O, 40 mg CaCl_2,20 mg FeSO_4・7H_2O, 20 mg MnSO_4・4-6H_2O, and 20 mg CuSO_4・5H_2O in 1 l(pH 5.5).
社団法人日本生物工学会の論文
1978-10-25