Production of Benzoylformic Acid from Phenylglycine by Saccharomycopsis lipolytica(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
Microbial production of benzoylformic acid (BF), which can be used as a substrate of enzymatic synthesis of (R)-(-)-mandelic acid, was investigated. Among 145 strains of yeasts and actinomycetes, Saccharomycopsis lipolytica (IAM 4964) was the best producer of BF from DL-phenylglycine (DL-PG). Culture conditions for BF production by the organism were optimized. When 0.2 % fructose as a carbon source and 0.7 % Bacto-tryptone as a nitrogen source were used in the presence of 4 % DL-PG, 14.5 mg/ml of BF was produced (about 37 % molar yield) in 4 days of cultivation. BF was synthesized from the L-form of PG, but not from the D-form. The BF was isolated from culture broth in a crystalline form and physicochemically identified.
- 社団法人日本農芸化学会の論文
- 1990-08-23
著者
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Kanzaki Hiroshi
Department of Biofunctional Chemistry, Okayama University Graduate School of Natural Science and Tec
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YAMADA Hideaki
Department of Agricultural Chemistry, Kyoto University
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Isobe Atsuhiko
Department Of Agricultural Chemistry Kyoto University
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Izumi Yoshikazu
Department Of Agricultural Chemistry Kyoto University
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Izumi Yoshikazu
Department Of Agricultural Chemistry Kyoto University:(present Office)department Of Biotechnology To
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Kanzaki Hiroshi
Department Of Agricultural Chemistry Kyoto University:(present Office)division Of Bio-resources Scie
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Yamada Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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