Involvement of Flavin Coenzyme in Dibenzothiophene Degrading Enzyme System from Rhodococcus erythropolis D-1
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概要
- 論文の詳細を見る
In the process of the purification of a dibenzothiophene (DBT)-degrading enzyme system from cell-free extracts of Rhodococcus erythropolis D-1, flavin coenzymes, FMN and FAD, were found to be involved in the enzymatic degradation of DBT in addition to NADH. Under these experimental conditions, the optimal concentrations of FMN and FAD were both 10 μM and the activity was completely inhibited by adding 1 mM FMN or FAD. DBT was converted to DBT sulfone stoichiometrically and 2-hydroxybiphenyl formation was not observed when the reaction was done using the enzyme preparation purified by DEAE-Sepharose column chromatography.
- 社団法人日本農芸化学会の論文
- 1995-07-23
著者
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Ohshiro Takashi
Department Of Biotechnology Faculty Of Engineering Tottori University
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Izumi Yoshikazu
Department Of Agricultural Chemistry Kyoto University
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Izumi Yoshikazu
Department Of Biotechnology Faculty Of Engineering Tottori University
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Kanbayashi Yoshiaki
Department of Biotechnology, Faculty of Engineering, Tottori University
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Hine Yoshimitsu
Department of Biotechnology, Faculty of Engineering, Tottori University
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Hine Yoshimitsu
Department Of Biotechnology Faculty Of Engineering Tottori University
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Kanbayashi Yoshiaki
Department Of Biotechnology Faculty Of Engineering Tottori University
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