Expression of the β-D-Glucosidase I Gene in Bifidobacterium breve 203 during Acclimation to Cellobiose

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概要

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• The β-glucosidase I(EC 3.2.1.21)activity of Bifidobacterium breve 203 isokated from feces of adult humans was found to be verty low, but when B. breve 203 was acclimated to cellobiose, the activity of the enzyme increased in the acclimated cell, B. breve 203 cld. With the aim of elucidating the mechanism, responsible for this increased activity the translation and transcription of the β-D-glucosidase I gene were investigated using an antibody against β-D-glucosidase I and a probe specific to the gene, respectively. Western and Norther blots showed marked differeces in the expression of the enzyme and the level of its mRNA between B. breve 203 and B. breve 203 clb. However, genomic Southern blots revealed only insignificant differences in the copy numbers of the open reading frames in the two strains. The 5' flanking regions of the β-D-glucosidase I gene of B. breve 203 and tha of B. breve 203 clb were therefore analyzed. The transcriptional strat point, located 94 bp upstream from the first methionine codon, was identified by primer externsion. A sequence, -TTGGAA-(15 bp)-TAATCT-, located 8 bp upstream from the transcriptional strat point, was assigned as the promoter of the gene. A sequence highly homoologous to the lac operator region of Escherichia coli was found downstream of the transcriptional strat point.

• 社団法人日本生物工学会の論文
• 1997-04-25

著者

• KUMAGAI HIDEHIKO

  Department of Food Science and Technology, Kyoto University

• OHDAN Kohji

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• Kumagai Hidehiko

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Department Of Food Science And Technology Facylty Of Agriculture Kyoto University

• NUNOURA AOKI

  Department of Food Science and Technology, Facylty of Agriculture, Kyoto University

• YMAMOTO KENJI

  Department of Food Science and Technology, Facylty of Agriculture, Kyoto University

• Nunoura Aoki

  Department Of Food Science And Technology Facylty Of Agriculture Kyoto University

• Ohdan Kohji

  Department Of Food Science And Technology Facylty Of Agriculture Kyoto University

• Ymamoto Kenji

  Department Of Food Science And Technology Facylty Of Agriculture Kyoto University

• Nunoura Naoki

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University Kitashirakawa

• Yamamoto Kenji

  Department Of Cardiovascular Surgery Kanagawa Cardiovascular And Respiratory Center

• KUMAGAI Hidehiko

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

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