Synthesis of γ-Glutamyl-DOPA from L-Glutamine and L-DOPA by γ-Glutamyltranspeptidase of Escherichia coli K-12(Microbilolgy & Fermentation Industry)

概要

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An E. coli strain, SH209, harboring pLC9-12 exhibited 5- to 6-fold higher γ-glutamyltranspeptidase activity than the wild-type strain, at each growth temperature tested. Maximum activity was observed at 20〜25℃, as was observed with the wild type. A homogeneous enzyme preparation was obtained from the periplasmic fraction of the strain by a simple three-step method. The conditions for γ-glutamyl-DOPA synthesis form L-glutamine and L-DOPA were investigated using the enzyme preparation. Under the best conditions, the maximal yield of 79%, equivalent to 158mM (51.5g/l) of γ-glutamyl-DOPA as to both substrates, was obtained, γ-Glutamyl-DOPA was isolated from the reaction mixture and identified using an amino acid analyzer after hydrolysis with HCl or γ-glutamyltranspeptidase.
社団法人日本農芸化学会の論文
1988-07-23