Purification, Characterization, and Crystallization of Monoamine Oxidase from Escherichia coli K-12

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概要

• 論文の詳細を見る

• The gene for monoamine oxidase (MAO) was cloned from an Escherichia coli genomic library and MAO was overproduced in the periplasmic space. The enzyme was purified to homogeneity by preparation of a periplasmic fraction, followed by ammonium sulfate fractionation and DEAE-cellulose column chromatography. Crystals were obtained by the hanging drop method using sodium citrate as a precipitant. The enzyme was found to be a dimer of identical subunits with a molecular weight of 80,000, and showed the highest activity at pH 7.5 and 45℃. The enzyme was inhibited by a MAO specific inhibitor, hydroxylamine, hydrazine, phenelzine, isoniazid, and tranycypromine. The enzyme oxidized tyramine, phenethylamine, and tryptamine at higher rates, but not oxidized diamine and polyamines such as putrecine and spermine. The antibody against E. coli MAO cross-reacted with purified MAO A from Klebsiella aerogenes.

• 社団法人日本農芸化学会の論文
• 1994-09-23

著者

• YAMASHITA MITSUO

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• MUROOKA YOSHIKATSU

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• Yamashita Mitsuo

  Department Of Biotechnology Graduate School Of Engineering Osaka University:department Of Applied Ch

• Yamashita M

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Murooka Yoshikatsu

  Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T

• Murooka Y

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kumagai H

  Kyoto Univ. Kyoto Jpn

• KUMAGAI HIDEHIKO

  Department of Food Science and Technology, Kyoto University

• SUZUKI Hideyuki

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• Kumagai H

  Kyoto Univ.

• Kumagai H

  Nihon Univ. Fujisawa‐shi Jpn

• Kumagai Hidehiko

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Roh Jung

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• Azakami Hiroyuki

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• Roh Jung

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Division Of Integrated Life Sciences Graduate School Of Biostudies Kyoto University

• Suzuki H

  Kyoto Univ. Kyoto Jpn

• Suzuki H

  The United Graduate School Of Agricultural Science Gifu University

• Azakami H

  Department Of Biotechnology Graduate School Of Engineering Osaka University:school Of Dentistry Toku

• Azakami Hiroyuki

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Murooka Yoshikatsu

  Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.

• Matsumoto Yukimasa

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Suzuki Hideyuki

  Department Of Environmental And Ocean Engineering University Of Tokyo

• Yamashita Mitsuo

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol

• Suzuki Hideyuki

  Department Of Biological Science And Technology Science University Of Tokyo

• Suzuki Hideyuki

  Department Of Bio-medical Engineering School Of High-technology For Human Welfare Tokai University

• KUMAGAI Hidehiko

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

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