AsNODc22, a Novel Nodulin Gene of Astragalus sinicus, Encodes a Protein that Localizes along the Cell Wall of Bacteria-Infected Cells in a Nodule

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概要

• 論文の詳細を見る

• A novel nodulin gene, AsNODc22, was isolated from Astragalus sinicus cv.Japan (Renge-sou) by differential screening. The transcript of AsNODc22 was nodule specific, and its size was approximately 0.8 knt. AsNODc22 encoded an unknown ORF containing a putative signal peptide and a pair of similar hepta-peptides (CSAQVSC, CSAQLSC). For immunological study, antiserum against AsNODc22 protein was prepared in mice. By immunoblot analysis, the antiserum detected a nodule specific band at approximately 18 kDa. Immunofluorescence microscopy was used to observe localization of the gene product in the nodule. The signals were seen along the cell wall of bacteria-infected cells, but no significant signals were seen on uninfected cells.

• 日本植物生理学会の論文

著者

• Fujie Makoto

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Yamada Takashi

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• MUROOKA YOSHIKATSU

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• Murooka Yoshikatsu

  Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T

• Murooka Y

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• NAKANISHI YUJI

  Gifu Research and Development Center, Amano Enzyme Inc.

• Fujie M

  Graduate School Of Advanced Sciences Of Matter Hiroshima University

• Fujie M

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Fujie Makoto

  Department Of Molecular Biotechnology Graduate School Of Engineering Hiroshima University

• NAKANISHI YASUAKI

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• Nakanishi Yasuaki

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Fujie Makoto

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Yamada Takashi

  Department Of Chemistry Faculty Of Science And Engineering Konan University

• Nakanishi Yuji

  Gifu Research And Development Center Amano Enzyme Inc.

• Yamada T

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Murooka Yoshikatsu

  Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.

• Yamada Takashi

  Department Of Animal Science Graduate School Of Agricultural Science Kobe University

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