Use of lac Gene Fusions to Study Regulation of Tyramine Oxidase, Which is Involved in Derepression of Latent Arylsulfatase in Escherichia coli

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概要

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• Strains with lac fused to each of the arylsulfatase (ats) and tyramine oxidase (tyn) operons in Escherichia coli were isolated. Synthesis of β-galactosidase in strains with tyn : :lac fusions was induced by tyramine, histamine, tryptamine, dopamine and octopamine, and the induction of the tyn operon was subject to catabolite and ammoniumrepressions. These repressions were relieved whenthe cells were grown with a poor carbon or nitrogen source. No arylsulfatase activity is detected in E. coli strains. Synthesis ofβ-galactosidase in strains with ats : : lac fusions was repressed by sulfur compounds. The repression was relieved by monoamine compounds, which induced tyramine oxidase synthesis. The inhibition of tyramine oxidase activity by cysteine resulted in a decrease of the derepressed synthesis of β-galactosidase in the ats : : lac fusion. Repressing and derepressing conditions for the tyn operon prevented and stimulated, respectively, expression of the ats operon. Thus, the expression of latent arylsulfatase in E. coli seems to be regulated by expression of the tyn operon.

• 社団法人 日本農芸化学会の論文

著者

• MUROOKA YOSHIKATSU

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• Murooka Yoshikatsu

  Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T

• YAMASHITA Mitsuo

  The Insitute of Scientific and Industrial Research, Osaka University

• Murooka Yoshikatsu

  Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.

• MUROOKA Yoshikatsu

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

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