Development of a Host-Vector System for Lactobacillus plantarum L137 Isolated from a Traditional Fermented Food Produced in the Philippines
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概要
- 論文の詳細を見る
Lactobacillus plantarum NC13, a strain derived from the L.plantarum strain L137 isolated from a traditional fermented food produced in the Philippines, contains 9 of the 15 plasmids in the parental strain. To construct a shuttle vector between L.plantarum and Escherichia coli for genetic manipulation of L137 and its derivatives, recombinant plasmids were prepared by using the 9-plasmid DNA mixture and an E.coli vector, pBluescript II SK+. The resultant recombinant plasmids were retransferred to L.plantarum NCL21, an NC13-derived strain cured of 3 of the 9 plasmids, and 3 recombinant plasmids were obtained. The smallest plasmid, pRN14, contained a small cryptic plasmid, pLTK2, which is one of the plasmids in L.plantarum L137. Thus, the complete nucleotide sequence of pLTK2 was determined. The pLTK2 is 2295 bp in length, and has a major open reading frame of 951 bp. An encoded sequence of 317-amino acids showed extensive similarity with genes encoding replication protein (repA). A putative replication origin in pLTK2 also showed high homology to those of other gram-positive bacterial plasmids that replicate by the rolling circle mechanism. The shuttle vector pRN14 contained the erythromycin resistance gene and the ColE1 and pLTK2 replication origins. Transformation of L.plantarum strains with pRN14 by electroporation was optimized to give a transformation efficiency of 2×10^4 transformants/μg plasmid. Plasmid pRN14 was stably maintained in strain NCL21, as well as in L.casei K95-5.
- 社団法人日本生物工学会の論文
- 2000-01-25
著者
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Kaneko Yoshinobu
Department of Biotechnology, Graduate School of Engineering, Osaka University
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MUROOKA YOSHIKATSU
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech
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Murooka Yoshikatsu
Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T
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Murooka Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ono Hisayo
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KIATPAPAN PORNPIMON
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KOBAYASHI HAJIME
Department of Biotechnology, Graduate School of Engineering, Osaka University
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NISHIMOTO TAKAYUKI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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NAPITUPULU ROSTIATI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Ono Hisayo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ono H
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ono Hisayo
Department Of Biotechnology Faculty Of Engineering Osaka University
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Napitupulu Rostiati
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kiatpapan P
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Address)departm
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Kiatpapan Pompimon
Rangsit大・理・生化
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Kaneko Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kaneko Yoshinobu
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kaneko Yoshinobu
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
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Murooka Yoshikatsu
Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.
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Kiatpapan Pornpimon
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Address)departm
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Nishimoto Takayuki
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kobayashi Hajime
Department Of Agricultural Information Science Faculty Of Agriculture Tottori University
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KANEKO Yoshinobu
Department of Biotechnoiogy, Graduate School of Engineering, Osaka University
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