Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

スポンサーリンク

概要

• 論文の詳細を見る

• Expression profiles of protein phosphatase (PPase) disruptants were analyzed by use of Pearson's correlation coefficient to find profiles that correlated with those of 316 Reference Gene (RG) disruptants harboring deletions in genes with known functions. Twenty-six Δppase disruptants exhibited either a positive or negative correlation with 94 RG disruptants when the p value for Pearson's correlation coefficient was >0.2. Some of the predictions that arose from this analysis were tested experimentally and several new Δppase phenotypes were found. Notably, Δsit4 and Δsiw14 disruptants exhibited hygromycin B sensitivity, Δsit4 and Δptc1 disruptants grew slowly on glycerol medium, the Δptc1 disruptant was found to be sensitive to calcofluor white and congo red, while the Δppg1 disruptant was found to be sensitive to congo red. Because on-going analysis of expression profiles of Saccharomyces cerevisiae disruptants is rapidly generating new data, we suggest that the approach used in the present study to explore PPase function is also applicable to other genes.

著者

• Hirasaki Masataka

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Nakamura Fumika

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Yamagishi Kazuo

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Numamoto Minori

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Shimada Yukiko

  Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

• Uehashi Keigo

  Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

• Muta Shigeru

  Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

• Sugiyama Minetaka

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kaneko Yoshinobu

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kuhara Satoru

  Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

• Harashima Satoshi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kuhara Satoru

  Department Of Genetic Resources Technology Faculty Of Agriculture Kyushu University

• Kuhara Satoru

  Department Of Agricultural Chemistry

• Harashima S

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Harashima Satoshi

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Kaneko Y

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kaneko Yoshinobu

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Muta Shigeru

  Department Of Genetic Resources Technology Faculty Of Agriculture Kyushu University

• Uehashi Keigo

  Department Of Genetic Resources Technology Faculty Of Agriculture Kyushu University

• Shimada Yukiko

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• Yamagishi Kazuo

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Numamoto Minori

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kaneko Yoshinobu

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Harashima Satoshi

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Hirasaki Masataka

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Hirasaki Masataka

  Department Of Bioresources Faculty Of Agriculture Ehime University

• Sugiyama M

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Sugiyama Minetaka

  Department Of Biochemical Engineering & Science Kyushu Institute Of Technology

• Nakamura Fumika

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Minetaka Sugiyama

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• KANEKO Yoshinobu

  Department of Biotechnoiogy, Graduate School of Engineering, Osaka University

• HARASHIMA Satoshi

  Department of Biotechnoiogy, Graduate School of Engineering, Osaka University

関連論文

• Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Development of a Host-Vector System for Lactobacillus plantarum L137 Isolated from a Traditional Fermented Food Produced in the Philippines
• Molecular Cloning of the Glucoamylase I Gene of Aspergillus awamori var. kawachi for Localization of the Raw-starch-affinity Site(Microbiology & Fermentation Industry)
• Application of the Bio-Active Beads Method in Rice Transformation
• An Arabidopsis thaliana Gene on the Yeast Artificial Chromosome Can Be Transcribed in Tobacco Cells
• Molecular Cloning and Expression of Uricase Gene from Arthrobacter globiformis in Escherichia coli and Characterization of the Gene Product(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Characterization of Nuclear, and Nuclear Matrix-Bound DNA Polymerases of Normal Rat Liver
• Purification and Characterization of an Acid Protease from Myxoamoebae of a True Slime Mold, Physarum polycephalum
• Fatty Acid Species of Bio-Active Lysophosphatidic acid from Haploid Myxoamoebae of Physarum Polycephalum
• Extracellular Polysaccharide from a True Slime Mold, Physarum Polycephalum. Polymerization and Auto-Degradation Associated with Cell Growth, and Its Inhibitory Activity on Eukaryotic DNA Polymerase α
• 3P-1006 Characterization of a suppressor of Δrrn10 disruptant causing the defect in rRNA transcription in Saccharomyces cerevisiae
• 2Bp14 Molecular breeding of Saccharomyces cerevisiae strain with high amount of RNA
• A Novel Gene Delivery System in Plans with Calcium Alginate Micro-Beads
• A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads(PLANT BIOTECHNOLOGY)
• Accumulation of Compatible Solutes, Ectoine and Hydroxyectoine, in a Moderate Halophile, Halomonas elengata KS3 Isolated from Dry Salty Land in Thailand
• Obtaining transgenic plants using the bio-active beads method
• Estimation of Cell Biomass in Plant Cell Suspensions by the Osmotic Pressure Measurement of Culture Broth
• Phylogenetic Position of Mesorhizobium huakuii subsp.rengei, a Symbiont of Astragalus sinicus cv.Japan
• 1056 Intraspesific variation of Mesorhizobium strains isolated from Astragalus sinicus cv. Japan
• Characterization of Starch-Hydrolyzing Lactic Acid Bacteria Isolated from a Fermented Fish and Rice Food, "Burong Isda", and Its Amylolytic Enzyme
• Characterization of the ATF1 and Lg-ATF1 Genes Encoding Alcohol Acetyltransferases in the Bottom Fermenting Yeast Saccharomyces pastorianus
• A Structural and Phylogenetic Study of the HO Gene from Saccharomyces bayanus var. uvarum
• Fatty Acid Desaturation in Methylotrophic Yeast Hansenula polymorpha Strain CBS 1976 and Unsaturated Fatty Acid Auxotrophic Mutants
• Effects on N-Glycosylation and Inositol on the ER Stress Response in Yeast Saccharomyces cerevisiae
• Mass Mating Method in Combination with G418- and Aureobasidin A-Resistance Markers for Efficient Selection of Hybrids from Homothallic Strains in Saccharomyces cerevisias
• Genes in PHT Plasmid Encoding the Initial Degradation Pathway of Phthalate in Pseudomonas putida
• 3P-1005 Physiological characterization of high-temperature tolerant Saccharomyces cerevisiae strain and identification of a responsible gene
• Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Creating a Saccharomyces cerevisiae Haploid Strain Having 21 Chromosomes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• 1102 Cloning and functional analysis of Δ^9-fatty acid desaturase gene of Hansenula polymorpha
• Efficient Production of Chitinase by Immobilized Wasabia japonica Cells in Double-Layered Gel Fibers
• Bacterial Alginate Lyase : Properties of the Enzyme Formed in a Mixed Culture of Bacteria Isolated from Soil
• Symbiotic Formation of Alginate Lyase in Mixed Culture of Bacteria Isolated from Soil
• Use of the PDR4 Gene as a Dominant Selective Marker in Combination with Cerulenin for Prototrophic Strains in Sacchromyces cerevisiae
• A Method for Direct Selection of Mating-Competent Clones from Mating-Incompetent Industrial Strains of Saccharomyces cerevisiae
• A System for Temperature-Controlled Expression of a Foreign Gene with Dual Mode in Saccharomyces cerevisiae
• 159 Four XY females found at the time of surgery for inguinal hernia
• Salt Shock Enhances the Expression of ZrATP2, the Gene for the Mitochondrial ATPase β Subunit of Zygosaccharomyces rouxii(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• A Novel 66-kDa Stress Protein,p66,Associated with the Process of Cyst Formation of Physarum polycephalum Is a Physarum Homologue of a Yeast Actin-Interacting Oritein,AIP1
• Improvement and Application of a Promoter-Probe Vector Bearing the PHO5 Gene as the Indicator Marker in Saccharomyces cerevisiae
• Partial Restoration of Sporulation Defect in Sake Yeasts, Kyokai No. 7 and No. 9,by Increased Dosage of the IME1 Gene
• EXPRESSION OF TELOMERASE ACTIVITY ASSOCIATED WITH TRANSFORMATION OF HUMAN FIBROBLASTS
• TELOMERE AND TELOMERASE IN HUMAN LEUKOCYTES AND IMMORTAL CELL LINES
• The Transcriptional Activators of the PHO Regulon, Pho4p and Pho2p, Interact Directly with Each Other and with Components of the Basal Transcription Machinery in Saccharomyces cerevisiae^1
• 1019 Cloning and characterization of Δ 9-fatty acid desaturase genes from Pichia, Yarrowia and Kluyveromyces
• Expression of a 66-kD Heat Shock Protein Associated with the Process of Cyst Formation of a True Slime Mold, Physarum polycephalum.
• A Simple PCR Method for Distinguishing Saccharomyces cerevisiae from Its Sibling species by Amplification of the RPL2 Region
• PHT, a Transmissible Plasmid Responsible for Phthalate Utilization in Pseudomonas putida
• A Simple Method for Detection of Enzyme Activities Involved in the Initial Step of Phthalate Degradation in Microorganisms
• 556 Cloning and functional analysis of FAS1 gene encoding fatty acid synthetase in Hansenula polymorpha
• Genetic Characterization of rbt Mutants That Enhance Basal Transcription from Core Promoters in Saccharomyces cerevisiae^1
• Control of Secretory Production of Human Lysozyme from Saccharomyces cerevisiar by Incubation Temperature and Phosphate Concentration
• Identification and Functional Characterization of Yeast ζ-COP
• Saccharomyces cerevisiae protein phosphatase Ppz1 and protein kinases Sat4 and Ha15 are involved in the control of subcellular localization of Gln3 by likely regulating its phosphorylation state(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Application of the PHO5-Gene-Fusion Technology to Molecular Genetics and Biotechnology in Yeast
• A Method for Fusing Chromosomes in Saccharomyces cerevisiae
• One-Step Splitting of a Chromosome in Haploid Cells of Saccharomyces cerevisiae and Its Effect on the Cell Proliferation
• One-step splitting of a chromosome in haploid cells of Saccharomyces cerevisiae
• A Structural and Phylogenetic Study of the HO Gene from Saccharomyces bayanus var. uvarum
• Strategy for preventing bacterial contamination by adding exogenous ethanol in solid-state semi-continuous bioethanol production(BIOCHEMICAL ENGINEERING)
• Construction of a Saccharomyces cerevisiae strain with a high level of RNA(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Tetraploid Formation through the Conversion of the Mating-type Alleles by the Action of Homothallic Genes in the Diploid Cells of Saccharomyces Yeasts
• A Yeast Artificial Chromosome-Splitting Vector Designed for Precise Manipulation of Specific Plant Chromosome Region(Genetics, Molecular Biology, and Gene Engineering)
• Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae
• Modification of Metabolic Pathways of Saccharomyces cerevisiae by the Expression of Lactate Dehydrogenase and Deletion of Pyruvate Decarboxylase Genes for the Lactic Acid Fermentation at Low pH Value
• Saccharomyces sensu stricto : Systematics, Genetic Diversity and Evolution
• 2Hp11 Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
• 1Fp06 Supraoptimal temperatures tolerance of Saccharomyces cerevisiae can be achieved by over-expression of RSP5 gene encoding ubiquitin ligase
• Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid homeostasis in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Large-scale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of mini-chromosomes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid homeostasis in Saccharomyces cerevisiae
• Genetic interactions of ribosome maturation factors Yvh1 and Mrt4 influence mRNA decay, glycogen accumulation, and the expression of early meiotic genes in Saccharomyces cerevisiae
• Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae
• Large-scale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of mini-chromosomes
• Enhanced bio-ethanol production from cellulosic materials by semi-simultaneous saccharification and fermentation using high temperature resistant Saccharomyces cerevisiae TJ14(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning
• Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Long-chain Phosphates. I. Reactions of Solid Potassium Kurrol Salt with Solutions of Various Metal Salts
• Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits
• Increased transcription of RPL40A and RPL40B is important for the improvement of RNA production in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Suppression mechanism of the calcium sensitivity in Saccharomyces cerevisiae ptp2Δmsg5Δ double disruptant involves a novel HOG-independent function of Ssk2, transcription factor Msn2 and the protein kinase A component Bcy1(GENETICS, MOLECULAR BIOLOGY, AND
• Single Nucleotide Polymorphism Analysis of a Trichoderma reesei Hyper-Cellulolytic Mutant Developed in Japan
• Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration
• Enhanced bio-ethanol production from cellulosic materials by semi-simultaneous saccharification and fermentation using high temperature resistant Saccharomyces cerevisiae TJ14

もっと見る 閉じる

スポンサーリンク