Extracellular Polysaccharide from a True Slime Mold, Physarum Polycephalum. Polymerization and Auto-Degradation Associated with Cell Growth, and Its Inhibitory Activity on Eukaryotic DNA Polymerase α

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概要

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• Plasmodia of a true slime mold, Physarum polycephalum, synthesize slime polysaccharide and secrete it outside the organisms. This substance was shown to be synthesized and polymerized with cell growth. And when the cells reached a plateau of growth in a nutrient medium, degradation of the polysaccharide and autolysis of the cells occurred. The composition of polysaccharides from different strains of Physarum, F/J or Lu strains, were almost the same, but polymerization rates of them were different. We purified the polysaccharides from these two strains and characterized them. Both of them mainly consisted of galactose (Gal) residue having phosphate (-PO_4) and sulfate (-SO_4) moieties, and the molar ratios of these constituents were 9:1:1 for F/J and 9:2:1 for Lu, respectively. The biological effect of slime polysacchapide was examined and we found that it had a specific inhibitory activity on eukaryotic DNA polymerase α, and degree of inhibition was not dependent on the length of the polysaccharide.

著者

• Shimada Yukiko

  Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

• Murakami-Murofushi Kimiko

  Department of Biology, Faculty of Science, Ochanomizu University

• SHIODA Masaki

  Department of Biological Science, Faculty of Science, Kumamoto University

• Shimada Yukiko

  Depertment Of Biology Faculty Of Science Ochanomizu University

• Shioda M

  Depertment Of Physiological Chemistry And Nutrition Faculty Of Medicine University Of Tokyo

• Shioda M

  Kumamoto Univ. Kumamoto Jpn

• Shioda Masaki

  Department Of Biological Science Faculty Of Science Kumamoto University

• Shimada Yukiko

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• Ohta Jiro

  Department of Biology, Faculty of Science, Ochanomizu University

• Ohta J

  Department Of Biology Faculty Of Science Ochanomizu University

• Ohta Jiro

  Department Of Biology Faculty Of Science Ochanomizu University

• Murakami‐murofushi K

  Department Of Biology Fscutry Of Science Ochanomizu University

• Murakami-murofushi Kimiko

  Department Of Biology Faculty Of Science Ochanomizu University

関連論文

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• Fatty Acid Species of Bio-Active Lysophosphatidic acid from Haploid Myxoamoebae of Physarum Polycephalum
• Extracellular Polysaccharide from a True Slime Mold, Physarum Polycephalum. Polymerization and Auto-Degradation Associated with Cell Growth, and Its Inhibitory Activity on Eukaryotic DNA Polymerase α
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