RESPONSE OF A TRUE SLIME MOLD, Physarum polycephalum, TO SALT STRESS
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概要
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Under high salt conditions, haploid myxoamoebae of a true slime mold, Physanun polycephalum retract their pseudopodia and change their cell shape into disk-like form, after which they construct the cell wall to form their dormant type designated microcysts. We observed the change of the intracellular distribution of actin filaments associated with the cellular morphological changes. Staining with phalloidin showed that the actin filaments were almost uniformly distributed throughout the myxoamoebae cytoplasm. When these cells were exposed to salt stress, the actin structures changed into short rods or dots, and they localized along the periphery of the cell. An incubation of the myxoamoebae in high salt medium, caused the synthesis of several species proteins, among which a 66-kD protein (p66) was most prominently induced.We found that p66 was co-precipitated with polymerized actin and bound to ATP-agarose. A double staining of the disk-shaped cells with anti-p66 antibody and phalloidin revealed superimposable localization of p66 and actin filaments in the short rods or dots. And it was revealed that p66 was immunologically unrelated to the common heat shock protein,HSP70 and HSP90,those are highly conserved during evolution. From these results, p66 is supposed to be a novel stress protein and interact with actin to perticipate in the morphological changes of the cell induced by salt stress.
- お茶の水女子大学の論文
著者
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Uchiyama Naoko
Department of Biology, Faculty of Science, Ochanomizu University
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Uchiyama N
Ochanomizu Univ.
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Uchiyama Naoko
Department Of Biology Faculty Of Science Ochanomizu University
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Murakami-murofushi Kimiko
Department Of Biology Faculty Of Science Ochanomizu University
関連論文
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