Examination of The Correlation between DNA-dependent Protein Kinase and Telomerase Activity

スポンサーリンク

概要

• 論文の詳細を見る

• Telomerase is known as a ribonucleoprotein enzyme essential for the repair of replication-induced deletion of chromosome terminal in most eukaryotes. Although the regulatory mechanism of telomerase inactivation and re-activation has not yet been clarified, there is a possibility that telomerase activity is regulated by phosphorylation and dephosphorylation. DNA-dependent protein kinase (DNA-PK) is considered to be a critical enzyme in DNA double-strand break repair and possibility of involvement of this enzyme in the regulation of telomerase is expected. To examin correlation between telomerase and DNA-PK, we purified DNA-PK from human leukaemic MOLT-4 cells by successive column chromatography. Through the conventional process of purification, DNA-PK activity was accompanied by telomerase activity. We used modified purification protcol in this study, and tinaly, succeeded in purification of DNA-PK separately from telomerase activity. This DNA-PK didn't show any effect on the telomerase activity, and these results indicate that the telomerase may not be regulated via phosphorylation with DNA-PK.

• お茶の水女子大学の論文

著者

• Matsumoto Yoshihisa

  Department Of Anesthesiology And Intensive Care Medicine Graduate School Of Medical Science Kanazawa

• Murakami-murofushi Kimiko

  Department Of Biology Faculty Of Science Ochanomizu University

• Murakami-murofushi Kimiko

  Department Of Biology Faculty Of Sience Ochanomizu University

• Suzuki Noriko

  Department Of Biochemistry And Molecular Biology Division Of Gene Therapy Research Center For Advanc

• Suzuki Norio

  Department Of Biological Sciences Graduate School Of Science Hokkaido University

• Matsumoto Yoshihisa

  Department Of Radiation Oncology Faculty Of Medicine University Of Tokyo

• Ikeda Fumi

  Department of Biology, Faculty of Sience, Ochanomizu University

• Ikeda Fumi

  Department Of Biology Faculty Of Sience Ochanomizu University

• Suzuki Norio

  Department Of Radiation Oncology Faculty Of Medicine University Of Tokyo

• Suzuki Norio

  Department Of Agricultural Chemistry The University Of Tokyo

• Suzuki Noriko

  Department of Applied Pharmacology, Kyoto Pharmaceutical University

関連論文

• 2Cp16 CHO細胞における染色体の識別および染色体安定性の解析(動物細胞工学・動物組織培養,一般講演)
• 2I09-3 CHO細胞における染色体の識別及び再配列解析(動物細胞工学・動物組織培養,一般講演)
• 2P127 ミニサテライト反復DNA配列の高次構造形成と繰り返し数の変動(核酸 A) 構造・物性))
• 責任遺伝子の特定された副甲状腺機能低下症 : 自己免疫性多腺性内分泌不全症I型(APS1, APECED)
• 1型糖尿病, 特発性副甲状腺機能低下症に橋本病, 尋常性白斑を併発した多腺性自己免疫症候群の1例
• CHARACTERIZATION OF THE MEDAKA Y-CHROMOSOME SPECIFIC REGION(Genetics,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• The DNA Sequence of Medaka Chromosome LG22(Comparative Genomics of Animals,Symposium,PROCEEDING OF THE 76^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
• A novel membrane guanylyl cyclase expressed in medaka (Oryzias latipes) intestine.
• POLYMORPHISM OF THE MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I REGION OF MEDAKA, ORYZIAS LA TIPES(Taxonomy and Systematics,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• Sequence analysis of filaggrin gene by novel shotgun method in Japanese atopic dermatitis
• FOUR DISTINCT C-TYPE NATRIURETIC PEPTIDES FOUND IN MEDAKA REVEAL NEW EVOLUTIONARY HISTORY OF THE NATRIURETIC PEPTIDE FAMILY(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• 臨床遺伝子診断学研究の進歩と展望 遺伝子変異データベース (遺伝子診療学(第2版)--遺伝子診断の進歩とゲノム治療の展望) -- (遺伝子診断)
• 2-a) 遺伝子変異データベース構築のための情報収集と抽出の現状(バイオ研究者からのバイオNLPへの期待)(ポストゲノム時代に高まるバイオ自然言語処理への期待 : バイオ自然言語処理最新事情)
• 2I09-2 CHO細胞遺伝子増幅メカニズムの解明にむけた染色体上増幅領域の配列解析(動物細胞工学・動物組織培養,一般講演)
• Scale osteoblasts and osteoclasts sensitively respond to low-gravity loading by centrifuge
• ANALYSIS OF OSTEOBLAST SPECIFIC MESSENGER RNA EXPRESSION IN THE REGENERATING SCALE OF GOLDFISH, Carassius auratus
• MOLECULAR CLONING OF cDNAs ENCODING MARKERS IN OSTEOCLASTS, TRAP AND CATHEPSIN K IN GOLDFISH(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• α-Naphthylisothiocyanate Induces Intrahepatic Bile Duct with Greater Proliferation in Female Rats than in Males
• Impurity Behavior during the H-Mode in JFT-2M
• Ion Bernstein Wave Experiment on JFT-2M Tokamak
• Enhancement of Wall Fueling and Improvement in nτ of a Beam-Heated H-Mode Discharge by Electron Cyclotron Wave in the JFT-2M Tokamak
• Measurement of Peripheral Electron Temperature by Electron Cyclotron Emission during the H-Mode Transition in JFT-2M Tokamak
• Vasculoprotective effect of cilostazol in aldosterone-induced hypertensive rats
• Preliminary Evaluation of Toxicologic and Carcinogenic Risks of Copper Gluconate in Rats Given Multiple Carcinogens
• Increased in Vitro Release of Interferon-Gamma from Ampicillin-Stimulated Peripheral Blood Mononuclear Cells in Stevens-Johnson Syndrome(Biopharmacy)
• Detection of Weak Sugar Binding Activity of VIP36 using VIP36-streptavidin Complex and Membrane-based Sugar Chains
• Alloy Design of Nb-Based Hydrogen Permeable Membrane with Strong Resistance to Hydrogen Embrittlement
• Schottky Contact Characterization of Sputter-Etched Surface of GaAs and Its Recovery by Annealing
• RESPONSE OF A TRUE SLIME MOLD, Physarum polycephalum, TO SALT STRESS II : The Effects of The Inhibitors for RNA and Protein Syntheses on Stress-induced Microcyst Formation
• Systemic Distribution of Salusin Expression in the Rat
• Gene Analysis and Evaluation of the Single Founder Effect in Japanese Patients with Oguchi Disease
• Gallium Oxide Film by Anodic Oxidization of Gallium
• Characterization of Nuclear, and Nuclear Matrix-Bound DNA Polymerases of Normal Rat Liver
• Purification and Characterization of an Acid Protease from Myxoamoebae of a True Slime Mold, Physarum polycephalum
• Fatty Acid Species of Bio-Active Lysophosphatidic acid from Haploid Myxoamoebae of Physarum Polycephalum
• Extracellular Polysaccharide from a True Slime Mold, Physarum Polycephalum. Polymerization and Auto-Degradation Associated with Cell Growth, and Its Inhibitory Activity on Eukaryotic DNA Polymerase α
• TWO GUANYLYL CYCLASE C RECEPTORS AND ITS SELECTIVITY OF THREE LIGANDS, GUANYLIN, UROGUANYLIN AND RENOGUANYLIN, IN THE EEL(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• Dax1 REGULATES EXPRESSION OF P450arom IN MEDAKA FOLLICLES(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• CLONING AND ANALYSIS OF GONADAL EXPRESSION OF TESTICULAR TYPE SOX9 IN THE MEDAKA, ORYZIAS LATIPES(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• 胆道閉鎖症術後患児におけるMRIを用いた肝形態機能の経時的変化の検討
• EXPRESSION ANALYSIS OF TIMP-2 DURING OSTEOCLAST ACTIVATION IN THE SCALES OF GOLDFISH(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• Effects of the Anti-Platelet Aggregation Drug Dilazep on Cognitive Function in Dahl Salt-Sensitive Rats
• CHANGES OF MELATONIN CONCENTRATIONS IN THE SCALE OF FEMALE GOLDFISH DURING REPRODUCTIVE PERIOD(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• Sensitization by Wortmannin of Heat- or X-ray Induced Cell Death in Cultured Chinese Hamster V79 Cells
• Increased Expression after X-Irradiation of MUC1 in Cultured Human Colon Carcinoma HT-29 Cells
• A Gel-electrophoretic Analysis for Improved Sensitivity and Specificity of DNA-dependent Protein Kinase Activity
• Edge Pedestal During the H-Mode of a Tokamak Plasma Measured by the Electron Cyclotron Emission
• Physiologic Role of Decidual β_1 Integrin and Focal Adhesion Kinase in Embryonic Implantation
• ESTABLISHMENT OF EXPERIMENTAL MODEL FOR BONE DISEASE USING THE SCALE OF GOLDFISH, Carassius auratus
• Prurigo Pigmentosa on a Patient with Soft-Drink Ketosis
• Further Application and Evaluation of Critical Cell Number and Modifying Factors in Radiocurability of Multicellular Spheroids
• Identification of 66 kDa phosphoprotein associated with motility initiation of hamster spermatozoa
• Anaplastic Thyroid Carcinoma Associated with Granulocyte Colony-Stimulating Factor : Report of a Case
• Intraoperative Prediction of Compensatory Sweating for Thoracic Sympathectomy
• Long-term survival of a patient with advanced adenosquamous carcinoma of the gallbladder after radical resection
• Rectal adenocarcinoma metastatic to the thyroid gland
• The genome size evolution of medaka (Oryzias latipes) and fugu (Takifugu rubripes)
• The novel mutant scl of the medaka fish, Oryzias latipes, shows no secondary sex characters
• Radiation Biology, Theoretical strategy:How to make treatment planning for heavy ion-beam therapy ?
• Identification and Characterization of a Protein Found After X-Irradiation in Human T Cell Leukemia
• Thymidine Phosphorylase and Dihydropyrimidine Dehydrogenase Expression Levels in Tumor and Normal Tissue Specimens of T3 Human Colorectal Carcinoma
• INTRACELLULAR DAMAGE OF DNA INDUCED BY TRITIATED WATER AND ITS RESTORATION
• MRI Findings in the Liver in Biliary Atresia Patients after the Kasai Operation
• 3,4-Dihydroxyphenylalanine(DOPA) Decarboxylase Deficiency and Resultant High Levels of Plasma DOPA and Dopamine in Unfavorable Neuroblastoma
• Clinical outcome of percutaneous osteoplasty for pain caused by metastatic bone tumors in the pelvis and femur
• Long-term pain relief effects in four patients undergoing percutaneous vertebroplasty for metastatic vertebral tumor
• Steryl Glucoside is a Lipid Mediator in Stress-responsive Signal Transduction
• Synthesis of (-)-Allosamizoline, the Pseudoaminosugar Moiety of Allosamidin, a Chitinase Inhibitor
• EXPRESSION OF TELOMERASE ACTIVITY ASSOCIATED WITH TRANSFORMATION OF HUMAN FIBROBLASTS
• TELOMERE AND TELOMERASE IN HUMAN LEUKOCYTES AND IMMORTAL CELL LINES
• Inhibition of Cell Proliferation by a Unique Lysophosphatidic Acid, PHYLPA, Isolated from Physarum polycephalum: Signaling Events of Antiproliferative Action by PHYLPA
• Seizure recurrence after reduction of an antiepileptic drug in patients with unprovoked seizures and severe neurological abnormalities
• Effect of antiepileptic drugs on thyroid function
• Carbamazepine-induced thrombocytopenia and carbamazepine-10,11-epoxide : A case report
• Carbamazepine-exacerbated epilepsy with multifocal shifting independent epileptiform discharges on electroencephalogram : A case report
• STIMULATION OF Gq PROTEIN-COUPLED SIGNAL TRANSDUCTION BY CYCLIC PHOSPHATIDIC ACID (cPA) AND ACTIVATION OF PROTEIN KINASE C IN VITRO
• Expression of a 66-kD Heat Shock Protein Associated with the Process of Cyst Formation of a True Slime Mold, Physarum polycephalum.
• Selective Transduction of HIV-1-Infected Cells by the Combination of HIV and MMLV Vectors
• Activation of a Ca^ -dependent Protein Kinase in Response to Heat Shock in the Myxoamoebae of a True Slime Mold, Physarum Polycephalum
• Glycation of Myofibrillar Protein in Aged Rats and Mice
• Effects of Transforming Growth Factor Alpha and Hepatocyte Growth Factor on Acid-Induced Damage in Rat Gastric Epithelial Cells
• Effects of Sucralfate and Its Components on Acid- and Pepsin-Induced Damage to Rat Gastric Epithelial Cells
• Clinical Studies of Immunohistochemical Staining of DNA-dependent Protein Kinase in Oropharyngeal and Hypopharyngeal Carcinomas
• Intercalation of n-Alkylamines in Proton-Type Layered Titanium(IV) Oxide
• A Novel Purification Method of Sterols and Steryl Glucoside from Plasmodia of a True Slime Mold, Physarum Polycephalum
• RESPONSE OF A TRUE SLIME MOLD, Physarum polycephalum, TO SALT STRESS
• Biological Functions of a Novel Lipid Mediator, Cyclic Phosphatidic Acid (cPA)
• Examination of The Correlation between DNA-dependent Protein Kinase and Telomerase Activity
• Preservation of multifidusmuscle in the microlumbar disectomy
• Gimeracil, an inhibitor of dihydropyrimidine dehydrogenase, inhibits the early step in homologous recombination
• Inhibition of Aquaporin-3 Water Channel in the Colon Induces Diarrhea
• Assessment of Flow Quantity and Capture Efficiency of a Distal Protection Device
• The Relationship Between the Degree of Intraoperative Hypotension and the Onset of Postoperative Hypotension in Carotid Artery Stenting
• Cilostazol Reduces Periprocedural Hemodynamic Depression in Carotid Artery Stenting
• Histological Examination of Expanded Polytetrafluoroethylene Artificial Dura Mater at 14 Years After Craniotomy:—Case Report—
• Intracranial Tuberculoma in Non-immunosuppressive State:—Two Case Reports—
• Existence of poriferasterol monoglucoside and UDP-glucose: Poriferasterol glucosyltransferase in haploid myxoamoebae of mating-less mutant, Colonia strain, of Physarum polycephalum.
• Intracranial Tuberculoma in Non-immunosuppressive State -Two Case Reports-
• Cilostazol Reduces Periprocedural Hemodynamic Depression in Carotid Artery Stenting
• Histological Examination of Expanded Polytetrafluoroethylene Artificial Dura Mater at 14 Years After Craniotomy -Case Report-

もっと見る 閉じる

スポンサーリンク