Further Application and Evaluation of Critical Cell Number and Modifying Factors in Radiocurability of Multicellular Spheroids
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概要
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Relationship of cure and surviving clonogenic cell number after various doses of X-irradiation was examined in multicellular spheroids of LCT1 human lung adenocarcinoma cells, LCT2 human lung small cell carcinoma cells and FSA1233 mouse fibrosarcoma cells. Some of these spheroids were cured at such doses that considerable number of clonogenic cells still survived after irradiation. Radiocurability was analyzed by comparing total clonogenic cell number in spheroids, cellular radiosensitivity and critical cell number Nc,i.e., the minimum number of clonogenic cells required to produce regrowth. (Nc-1) cells were killed by post-radiation processes and the larger the critical cell number, the more radiocurable. The LCT2 spheroids had the largest critical cell number and were most radiocurable. To investigate underlying mechanisms, modifying effect of heavily irradiated (HIR) tumor cells on the clonogenicity,i.e., plating efficiency of unirradiated tumor cells was investigated. Plating efficiencies with HIR cells showed significant decrease in LCT2 cells, no change in LCTl cells and increase in FSA1233 cells. The results indicated that in case of LCT2 spheroids some of viable cells, surrounded by dying or dead cells might have been killed with unknown toxic effect additional to direct irradiation effect. Thus, critical cell number analysis may be useful to quantify and to compare modifying effect of cellular/environmental factors in curing process of spheroids or tumors.
- 日本放射線影響学会の論文
著者
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坂田 耕一
東京大学医学部放射線医学教室
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Suzuki Norio
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
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MAJIMA Hideyuki
Department of Oncology, Maxillofocal Radiology Division and Department of Space Environmental Medici
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Majima H
The Institute Of Industrial Science The University Of Tokyo
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OKADA SHIGEFUMI
Department of Radiation Oncology, Faculty of Medicine, University of Tokyo
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Okada S
Department Of Radiological Sciences Ibaraki Prefectural University Of Health Sciences
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Okada Shigefumi
Departemnt Of Radiation Biology Center Kyoto University
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Majima Hideyuki
Department Of Exp. Radiol. And Health Phys. Fukui Medical Univ.
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Majima Hideyuki
Department Of Mathematics Faculty Of Science Ochanomizu University
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Suzuki Norio
Department Of Biological Sciences Graduate School Of Science Hokkaido University
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Suzuki Norio
Department Of Environmental Biochemistry Graduate School Of Medicine Chiba University
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Suzuki Norio
Department Of Agricultural Chemistry The University Of Tokyo
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Majima Hideyuki
Department of Exp. Radiol. and health phys. , Fukui Medical Univ.
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