Sensitization by Wortmannin of Heat- or X-ray Induced Cell Death in Cultured Chinese Hamster V79 Cells

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概要

• 論文の詳細を見る

• Here we found that wortmannin sensitized Chinese hamster V79 cells to hyperthermic treatment at 44.0°C as determined either by colony formation assay or by dye exclusion assay. Wortmannin enhanced heat-induced cell death accompanying cleavage of poly (ADP-ribose) polymerases (PARP). Additionally, the induction of heat shock protein HSP70 was suppressed and delayed in wortmannintreated cells. Heat sensitizing effect of wortmannin was obvious at more than 5 or 10 μM of final concentrations, while radiosensitization was apparent at 5 μM. Requirement for high concentration of wortmannin, i.e., order of μM, suggests a possible role of certain protein kinases, such as DNA-PK and/ or ATM among PI3-kinase family. The sensitization was minimal when wortmannin was added at the end of heat treatment. This was similar to the case of X-ray. Since heat-induced cell death and PARP cleavage preceded HSP70 induction phenomenon, the sensitization to the hyperthermic treatment was considered mainly caused by enhanced apoptotic cell death rather than secondary to suppression or delay by wortmannin of HSP70 induction. Further, in the present system radiosensitization by wortmannin was also at least partly mediated through enhancement of apoptotic cell death.

• 日本放射線影響学会の論文

著者

• Suzuki N

  Dept. Radiat. Oncol. Grad. Sch. Med. Univ. Tokyo

• Suzuki Norio

  Department of Biological Sciences, Graduate School of Science, The University of Tokyo

• TOMITA Masanori

  Department of Radiation Oncology, Graduate School of Medicine, University of Tokyo

• Sakai K

  Resarch Center For Radiation Protection National Institute Of Radiological Sciences

• Tomita Masanori

  Radiation Safety Research Center Central Research Institute Of Electric Power Industry

• Suzuki Norio

  東京大学 医系研究 救急医

• MATSUMOTO Yoshihisa

  Department of Mechanical Engineering, Oita National College of Technology

• HIRANO Kazuya

  Lab. Public Health. Sch. Pharmacy and Life Science.

• HIRANO KAZUYA

  Department of Radiation Oncology, Graduate School of Medicine, University of Tokyo

• UMEDA NORIKO

  Department of Radiation Oncology, Graduate School of Medicine, University of Tokyo

• SAKAI KAZUO

  Department of Radiation Oncology, Graduate School of Medicine, University of Tokyo

• TOMITA Masanori

  RIKEN

• Tomita Masanori

  Department Of Radiation Oncology Graduate School Of Medicine University Of Tokyo

• Matsumoto Yukiko

  Radiat. Biol. Kyoto Univ.

• Matsumoto Yoshihisa

  Research Laboratory For Nuclear Reactors Tokyo Institute Of Technology

• Matsumoto Yoshihisa

  Department Of Anesthesiology And Intensive Care Medicine Graduate School Of Medical Science Kanazawa

• Matsumoto Yoshihisa

  Res. Lab. For Nuclear Reactors And Dep. Of Nuclear Engineering Tokyo Inst. Of Technol.

• Umeda Noriko

  Depts. Radiat. Res. And Radiat. Oncol. Grad. Sch. Med. Univ. Tokyo:tokyo Univ. Pharm. Life Sci.

• Sakata Koh-ichi

  Department Of Radiology Sapporo Medical University School Of Medicine

• Suzuki Norio

  Department Of Biological Sciences Graduate School Of Science Hokkaido University

• SAKAI Kazuo

  School of Law, Meiji University

• Sakai Kazuo

  Department Of Physics Faculty Of Science Science University Of Tokyo

• Matsumoto Yoshihisa

  Atomic Bomb Disease Institute Graduate School Of Biochemical Sciences Nagasaki University

• Suzuki Norio

  Department Of Agricultural Chemistry The University Of Tokyo

• Sakai Kazuo

  Department of Computer Science and Media Engineering, Faculty of Engineering, Yamanashi University

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