Free Radical Scavenger Edaravone Suppresses X-ray-induced Apoptosis through p53 Inhibition in MOLT-4 Cells

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概要

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• Edaravone, a clinical drug used widely for the treatment of acute cerebral infarction, is reported to scavenge free radicals. In the present study, we investigated the radioprotective effect of edaravone on X-ray-induced apoptosis in MOLT-4 cells. Apoptosis was determined by the dye exclusion test, Annexin V binding assay, cleavage of caspase, and DNA fragmentation. We found that edaravone significantly suppressed the X-ray-induced apoptosis. The amount of intracellular ROS production was determined by the chloromethyl-2', 7'-dichlorodihydro-fluorescein diacetate system. We found that the intracellular ROS production by X-irradiation was completely suppressed by the addition of edaravone. The accumulation and phosphorylation of p53 and the expression of p21^<WAF1>, a target protein of p53, which were induced by X-irradiation, were also suppressed by adding edaravone. We conclude that the free radical scavenger edaravone suppresses X-ray-induced apoptosis in MOLT-4 cells by inhibiting p53.

• 日本放射線影響学会の論文
• 2007-11-15

著者

• IGAKI Hiroshi

  Department of Radiology, Teikyo University School of Medicine

• Yoshioka Katsuji

  Nuclear Engineering Research Laboratory Graduate School Of Engineering The University Of Tokyo

• MIYAGAWA Kiyoshi

  Section of Radiation Biology, Graduate School of Medicine, The University of Tokyo

• NAKAGAWA Keiichi

  Department of Radiology, University of Tokyo Hospital

• SASANO Nakashi

  Department of Radiology, Graduate School of Medicine, The University of Tokyo

• ENOMOTO Atsushi

  Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medici

• HOSOI Yoshio

  Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medici

• KATSUMURA Yosuke

  Nuclear Engineering Research Laboratory, Graduate School of Engineering, The University of Tokyo

• MATSUMOTO Yoshihisa

  Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medici

• SHIRAISHI Kenshiro

  Department of Radiology, Graduate School of Medicine, The University of Tokyo

• Igaki Hiroshi

  Department Of Radiology Teikyo University School Of Medicine

• Matsumoto Yukiko

  Radiat. Biol. Kyoto Univ.

• Miyagawa Kiyoshi

  Department Of Radiation Biology University Of Tokyo Hospital

• Miyagawa Kiyoshi

  Section Of Radiation Biology Graduate School Of Medicine The University Of Tokyo

• Matsumoto Yoshihisa

  Research Laboratory For Nuclear Reactors Tokyo Institute Of Technology

• Matsumoto Yoshihisa

  Res. Lab. For Nuclear Reactors And Dep. Of Nuclear Engineering Tokyo Inst. Of Technol.

• Hosoi Yoshio

  Section Of Radiation Biology Center For Disease Biology And Integrative Medicine Faculty Of Medicine

• Igaki Hiroshi

  Department Of Radiation Oncology Tsukuba University Hospital University Of Tsukuba

• Sasano Nakashi

  Department Of Radiology Graduate School Of Medicine The University Of Tokyo:section Of Radiation Bio

• Enomoto Atsushi

  Section Of Radiation Biology Center For Disease Biology And Integrative Medicine Faculty Of Medicine

• Nakagawa Keiichi

  Department Of Radiology The University Of Tokyo Hospital

• Hosoi Yoshio

  Department Of Radiation Oncology University Of Tokyo School Of Medicine

• Nakagawa Keiichi

  Department Of Cardiovascular Science And Medicine

• Shiraishi Kenshiro

  Department Of Radiology University Of Tokyo Hospital

• Enomoto Atsushi

  Laboratory Of Molecular Radiology Center For Disease Biology And Integrative Medicine Graduate Schoo

• Katsumura Yosuke

  Department Of Nuclear Engineering And Management School Of Engineering The University Of Tokyo

• Katsumura Yosuke

  Nuclear Engineering Laboratory School Of Engineering University Of Tokyo

• Imamura Hiroshi

  Hepato-biliary-pancreatic Surgery Division Department Of Surgery Graduate School Of Medicine Univers

• Ikeda Hitoshi

  Department Of Clinical Laboratory Medicine The University Of Tokyo

• Nakagawa Keiichi

  東京大学医学部附属病院 放射線科

• Igaki Hiroshi

  Department Of Radiology Faculty Of Medicine University Of Tokyo

• Imamura Hiroshi

  Division Of Hepato-biliary-pancreatic Surgery Department Of Surgery Graduate School Of Medicine Univ

• Matsumoto Yoshihisa

  Atomic Bomb Disease Institute Graduate School Of Biochemical Sciences Nagasaki University

• Hosoi Yoshio

  Department Of Radiology University Of Tokyo Hospital:department Of Radiation Biology University Of T

• Igaki Hiroshi

  Department Of Clinical Laboratory The University Of Tokyo Hospital

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