Role of DNA Double-strand Break Repair Genes in Cell Proliferation under Low Dose-rate Irradiation Conditions
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概要
- 論文の詳細を見る
Radiation-induced DNA double-stand breaks (DSBs) lead to numerous biological effects. To elucidate the molecular mechanisms involved in cellular responses to low dose and low dose-rate radiation, it is informative to clarify the roles of DSB repair related genes. In higher vertebrate cells, there are at least two major DSB repair pathways, namely non-homologous end-joining (NHEJ) and homologous recombination(HR). Here, it is shown that in chicken DT40 cells irradiated with γ-rays at a low dose-rate(2.4cGy/day), the growth delay in NHEJ-related KU70- and PRKDC (encoding DNA-PKcs)-defective cells were remarkably higher than in cells defective for the HR-related RAD51B and RAD54 genes. DNA-PKcs-defective human M059J cells also showed an obvious growth delay when compared to control M059K cells. RAD54^<-/->KU70^<-/-> cells demonstrated their highest degree of growth delay after an X-irradiation with a high dose-rate of 0.9 Gy/min. However they showed a lower degree of growth delay than that seen in KU70^<-/-> and PRKDC^<-/-/-> cells exposed to low dose-rate irradiation. These findings indicate that cellular responses to low dose-rate radiation are remarkably different from those to high dose-rate radiation. The fact that both DT40 and mammalian NHEJ-defective cells were highly sensitive to low dose-rate radiation, provide a foundation for the concept that NHEJ-related factors may be useful as molecular markers to predict the sensitivity of humans to low dose-rate radiation.
- 2008-09-16
著者
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MATSUMOTO Yoshihisa
Research Laboratory for Nuclear Reactors and Department of Nuclear Engineering, Tokyo Institute of T
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Sakai K
Resarch Center For Radiation Protection National Institute Of Radiological Sciences
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Tomita Masanori
Radiation Safety Research Center Central Research Institute Of Electric Power Industry
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Sakai Kazuo
Resarch Center For Radiation Protection National Institute Of Radiological Sciences
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Otsuka Kensuke
Radiation Safety Res. Center Nuclear Technol. Res. Lab. Central Res. Inst. Of Electric Power Ind.
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TOMITA Masanori
RIKEN
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MOROHOSHI Fumiko
Radiation Safety Research Center, Central Research Institute of Electric Power Industry
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Matsumoto Yukiko
Radiat. Biol. Kyoto Univ.
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Matsumoto Yoshihisa
Research Laboratory For Nuclear Reactors Tokyo Institute Of Technology
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Matsumoto Yoshihisa
Research Laboratory For Nuclear Reactors And Department Of Nuclear Engineering Tokyo Institute Of Te
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Morohoshi Fumiko
Radiation Safety Research Center Central Research Institute Of Electric Power Industry
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Matsumoto Yoshihisa
Res. Lab. For Nuclear Reactors And Dep. Of Nuclear Engineering Tokyo Inst. Of Technol.
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Sakata Koh-ichi
Department Of Radiology Sapporo Medical University School Of Medicine
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Taguchi Mitsumasa
Radiation Safety Research Center Central Research Institute Of Electric Power Industry
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Taguchi Mitsumasa
Organic Pollutant Removal Technology Group Japan Atomic Energy Agency
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Otsuka Kensuke
電力中央研究所放射線安全研究センター
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Morohoshi Fumiko
Radiation Safety Res. Center Central Res. Inst. Of Electric Power Ind.
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SAKAI Kazuo
School of Law, Meiji University
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Matsumoto Yoshihisa
Atomic Bomb Disease Institute Graduate School Of Biochemical Sciences Nagasaki University
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Otsuka Kensuke
Radiat. Safety Res. Cent., CRIEP
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Tomita Masanori
Radiat. Safety Res. Cent., CRIEP
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