3'-Blocking Damage of DNA as a Mutagenic Lesion Caused by Hydrogen Peroxide in Escherichia coli

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概要

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• Ionizing radiation and hydrogen peroxide (H_2O_2 ) produce many types of oxidative DNA damage such as strand breaks, apurinic/apyrimidinic (AP) sites, base modifications and 3'-blocking damage such as phosphoglycolated and 3'-phosphorylated termini. AP sites and 3'-blocking damage are repairable by exonuclease III and endonuclease IV in Escherichia coli. XthA-nfo double mutants of E. coli, which are deficient in exonuclease III and endonuclease IV, were highly sensitive to lethal and mutagenic effects of H_2O_2 , compared with the wild-type strains. The pNT180 and pNT186 plasmids containing wild-type nfo and mutant nfo-186 gene, respectively, were introduced into the xthA-nfo mutant. The nfo-186 gene product, Nfo186, retained normal AP endonuclease activity but could not remove 3'-blocking damage from DNA. The pNT180 corrected the sensitivity of the xthA-nfo mutant to lethal and mutagenic effects of H_2O_2 . On the other hand, the pNT186 did not have any complementation effects. From these results it was concluded that 3'-blocking damage rather than an AP site is the primary lesion responsible for both lethal and mutagenic effects of H_2O_2 .

• 日本放射線影響学会の論文

著者

• ZHANG QIU-MEI

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• Zhang Q‐m

  Laboratory Of Radiation Biology Graduate School Of Science Kyoto University

• Zhang Q‐m

  Department Of Biological Sciences Graduate School Of Science Kyoto University

• YONEI Shuji

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• Takemoto Tadashi

  Department of Experimental Radiology, Shiga University of Medical Science

• IKEHATA HIRONOBU

  Department of Cell Biology, Graduate School of Medicine, Tohoku University

• MATSUMOTO YUKIKO

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• MITO SEIJI

  Research Laboratory, Tanabe Pharmaceutical Co. Ltd.

• IZUMI TADAHIDE

  Human Biological Chemistry and Genetics, University of Texas Medical Branch

• Takemoto Tadashi

  Department Of Experimental Radiology Shiga Medical University

• Takemoto Tadashi

  Kyoto Univ. Fac. Of Sci.

• Matsumoto Yukiko

  Radiat. Biol. Kyoto Univ.

• Matsumoto Yoshihisa

  Res. Lab. For Nuclear Reactors And Dep. Of Nuclear Engineering Tokyo Inst. Of Technol.

• Zhang Qiu-mei

  Laboratory Of Radiation Biology Graduate School Of Science Kyoto University

• Mito Seiji

  Research Laboratory Tanabe Pharmaceutical Co. Ltd.

• Yonei Shuji

  Laboratory Of Radiation Biology Graduate School Of Science Kyoto University

• Qiu-mei Zhang

  Kyoto Univ. Fac. Of Sci.

• Izumi T

  Department Of Communications And Computer Engineering Kyoto University

• Izumi Tadahide

  Human Biological Chemistry And Genetics University Of Texas Medical Branch

• Ikehata Hironobu

  Department Of Cell Biology Graduate School Of Medicine Tohoku University

• Ikehata Hironobu

  Department Of Radiation Research Tohoku University School Of Medicine

• Zhang Qiu-mei

  Lab. Of Radiat. Biol. Graduate School Of Science.

• Matsumoto Yoshihisa

  Atomic Bomb Disease Institute Graduate School Of Biochemical Sciences Nagasaki University

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