Induction of Repair Capacity for Oxidatively Damaged DNA as a Component of Peroxide Stress Response in Escherichia coli

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概要

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• We examined whether or not peroxide stress induces a repair capacity for oxidatively damaged DNA in Escherichia ccli cells. Peroxide stress was brought about by adding 30 μM hydrogen peroxide (H_2O_2) to exponentially growing cells. The following results were obtained. (1) After exposure to H_2O_2, E. ccli resistance to X-rays was enhanced. The acquisition of resistance was inhibited by rifampicin and chloramphenicol. (2) The response was acquired in mutants defective in the katG and oxyR genes, as well as in the wild-type strain. (3) Lambda phages damaged by exposure to H_2O_2 showed higher survival on H_2O_2-treated cells than on untreated cells. (4) The peroxide stress did not render E. ccli cells resistant to UV and mitomycin C. These suggest that peroxide stress induces a repair capacity against oxidative DNA damage and that this response must be regulated by a different mechanism from oxyR^+-mediated regulatory system.

• 日本放射線影響学会の論文

著者

• ZHANG QIU-MEI

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• Zhang Q‐m

  Department Of Biological Sciences Graduate School Of Science Kyoto University

• YONEI Shuji

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• MITO SEIJI

  Research Laboratory, Tanabe Pharmaceutical Co. Ltd.

• TAKEMOTO TADASHI

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• MITO SEIJI

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• Takemoto Tadashi

  Department Of Experimental Radiology Shiga Medical University

• Takemoto Tadashi

  Kyoto Univ. Fac. Of Sci.

• Zhang Qiu-mei

  Laboratory Of Radiation Biology Graduate School Of Science Kyoto University

• Mito Seiji

  Research Laboratory Tanabe Pharmaceutical Co. Ltd.

• Yonei Shuji

  Laboratory Of Radiation Biology Graduate School Of Science Kyoto University

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