Recombinant Schizosaccharomyces pombe Nth1 Protein Exhibits DNA Glycosylase Activities for 8-oxo-7,8-dihydroguanine and Thymine Residues Oxidized in the Methyl Group

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概要

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• Bacteria and eukaryotes possess redundant enzymes that recognize and remove oxidatively damaged bases from DNA through base excision repair. DNA glycosylases remove damaged bases to initiate the base excision repair. The exocyclic methyl group of thymine does not escape oxidative damage to produce 5-formyluracil (5-foU) and 5-hydroxymethyluracil (5-hmU). 5-foU is a potentially mutagenic lesion. A homolog of E. coli endonuclease III (SpNth1) had been identified and characterized in Shizosaccharomyces pombe. In this study, we found that SpNth1 recognizes and removes 5-foU and 5-hmU from DNA with similar efficiency. The specific activities for the removal of 5-foU and 5-hmU were comparable with that for thymine glycol. The expression of SpNth1 reduced the hydrogen peroxide toxicity and the frequency of spontaneous mutations in E. coli nth nei mutant. It was also revealed that SpNth1 had DNA glycosylase activity for removing 8-oxo-7, 8-dihydroguanine (8-oxoG) from 8-oxoG/G and 8-oxoG/A mispairs. These results indicated that SpNth1 has a broad substrate specificity and is involved in the base excision repair of 8-oxoG and thymine residues oxidized in the methyl group in S. pombe.

• 日本放射線影響学会の論文
• 2007-09-15

著者

• ZHANG QIU-MEI

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• Zhang Q‐m

  Laboratory Of Radiation Biology Graduate School Of Science Kyoto University

• Zhang Q‐m

  Department Of Biological Sciences Graduate School Of Science Kyoto University

• DOI Takashi

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• YONEI Shuji

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• Zhang Qiu-mei

  Department Of Biological Sciences Graduate School Of Science Kyoto University

• Doi Takashi

  Laboratory Of Radiation Biology Graduate School Of Science Kyoto University

• Yamamoto Kazuo

  Graduate School Of Life Sciences Tohoku Univ.

• YAMAMOTO Kazuo

  Department of Clinical Laboratory Medicine, Shiga University of Medical Science

• Yonekura Shin-Ichiro

  Laboratory of Stress Response Biology, Graduate School of Science, Kyoto University

• Sugiyama Hiroshi

  Laboratory Of Biological Chemistry Graduate School Of Science Kyoto University

• NAKAMURA Nobuya

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• NAKAMURA Nobuya

  Radiat. Biol. Grad. Sch. Sci. Kyoto Univ.

• Nakamura Nobuya

  Department Of Biological Sciences Graduate School Of Science Kyoto University

• Zhang Qiu-mei

  Laboratory Of Radiation Biology Graduate School Of Science Kyoto University

• Yonei S

  Department Of Biological Sciences Graduate School Of Science Kyoto University

• Yonei Shuji

  Labo. Radiat. Biol. Grad. Sch. Sci. Kyoto Univ.

• Yonei Shuji

  Laboratory Of Radiation Biology Graduate School Of Science Kyoto University

• Zhang Qiu

  Lab. Radiat. Biol. Grad. Sch. Sci. Kyoto Univ.

• Qiu-mei Zhang

  Kyoto Univ. Fac. Of Sci.

• Yonei Shuji

  Lab. Radiat. Biol. Grad. Sch. Sci. Kyoto Univ.

• Yonei Shuji

  Kyoto Univ. Fac. Of Sci.

• Yamamoto Kazuo

  Department Of Biomolecular Sciences Graduate School Of Life Sciences Tohoku University

• Zhang Qiu-mei

  Lab. Of Radiat. Biol. Graduate School Of Science.

• Yonekura Shin-ichiro

  Laboratory Of Stress Response Biology Graduate School Of Science Kyoto University

• Yonei Shuji

  Lab. Of Radiat. Biol. Graduate School Of Science.

• Yonei Shuji

  Kyoto Univ. Kyoto Jpn

• Yonekura Shin-Ichiro

  Laboratory of Radiation Biology, Graduate School of Science, Kyoto University

• Yamamoto Kazuo

  Department of Biology, Chiba University

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