A Novel Monofunctional DNA Glycosylase Activity Against Thymine Glycol in Mouse Cell Nuclei
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概要
- 論文の詳細を見る
Reactive oxygen species continuously oxidize DNA bases and threaten the genetic integrity. Thymine glycol (TG), one of the representative oxidized products, is repaired mainly by base excision repair (BER). In Escherichia coli, endonuclease III (Nth) and endonuclease VIII (Nei) are known to actively remove TG from DNA, and the homologs are well conserved in various organisms. These are bifunctional glycosylases, also associated with apurinic/apyrimidinic (AP) lyase activity. In the present study, a monofunctional TG-DNA glycosylase activity is shown to be one of the predominant nuclear activities present in some mouse tissues. By combining hypertonic extraction and column chromatography, we successfully separated the novel activity from majority of the bifunctional activities. Since it has been reported that mNTH1 may not be a dominant nuclear activity, the monofunctional glycosylase activity, together with mNEIL1, may be the major TG-DNA glycosylases in the mouse nucleus. The optimal reaction conditions for the monofunctional activity were found to be pH 7-8 and 100-150 mM KCl, and the activity was resistant to 20 mM EDTA. High monofunctional activity was detected in the spleen and stomach, while the level was significantly lower in the liver, suggesting that the contribution of the monofunctional activity is variable among organs.
- 日本放射線影響学会の論文
- 2008-05-16
著者
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MATSUYAMA Satoshi
Department of Precision Science and Technology, Graduate School of Engineering, University of Osaka
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KUBO KIHEI
Department of Radiology, School of Veterinary Medicine, University of Osaka Prefecture
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IDE Hiroshi
Grad. Dept. Math. Life Sci. Hiroshima Univ.
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Ide Hiroshi
Dep. Of Mathematical And Life Sciences Graduate School Of Sci. Hiroshima Univ.
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YAMAMOTO Kazuo
Department of Clinical Laboratory Medicine, Shiga University of Medical Science
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Ide Hiroshi
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Kubo K
Lab. Vet. Rradiol. Div. Sci. Graduate Sch. Agr. & Biol. Sci. Osaka Pref. Univ.
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Kubo Kihei
Department Of Advanced Pathobiology Graduate School Of Life & Environmental Sciences Osaka Prefe
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Kubo Kihei
大阪府立大学 生命環境科学部先端病態解析学
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Kubo Kihei
Department Of Veterinary Science Graduate School Of Agriculture And Biological Science Osaka Prefect
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Kubo Kihei
Laboratory Of Veterinary Radiology Department Of Veterinary Medicine College Of Agriculture Osaka Pr
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Kubo Kihei
Dept. Vet. Sci. Osaka Prefecture University
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YAMAMOTO Ryohei
Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefect
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AKIYAMA Miyuki
Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefect
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Yamamoto Ryohei
Department Of Advanced Pathobiology Graduate School Of Life & Environmental Sciences Osaka Prefe
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Akiyama Miyuki
Department Of Advanced Pathobiology Graduate School Of Life & Environmental Sciences Osaka Prefe
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Matuyama Satoshi
Department Of Advanced Pathobiology Graduate School Of Life & Environmental Sciences Osaka Prefe
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Matsuyama Satoshi
Vet. Sci. Grad. Sch. Agri. Biol. Sci. Osaka Pref Univ.
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Ide Hiroshi
Department Of Chemistry Faculty Of Science Kyushu University
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Yamamoto Kazuo
Department Of Biomolecular Sciences Graduate School Of Life Sciences Tohoku University
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Ide Hiroshi
Dept. Math. Life Sci. Hiroshima University
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Matsuyama Satoshi
Osaka Pref. Univ. School Of Vet. Med. Dept. Of Radiol.
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MATSUYAMA Satoshi
Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University
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Yamamoto Kazuo
Department of Biology, Chiba University
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