Quantitative Analysis of Isolated and Clustered DNA Damage Induced by Gamma-rays, Carbon Ion Beams, and Iron Ion Beams

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概要

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• Ionizing radiation induces multiple damaged sites (clustered damage) together with isolated lesions in DNA. Clustered damage consists of closely spaced lesions within a few helical turns of DNA and is considered to be crucial for understanding the biological consequences of ionizing radiation. In the present study, two types of DNA, supercoiled plasmid DNA and linear lambda DNA, were irradiated with γ-rays,carbon ion beams, and iron ion beams, and the spectra and yield of isolated DNA damage and bistranded clustered DNA damage were fully analyzed. Despite using different methods for damage analysis, the experiments with plasmid and lambda DNA gave largely consistent results. The spectra of both isolated and clustered damage were essentially independent of the quality of the ionizing radiation used for irradiation. The yields of clustered damage as well as of isolated damage decreased with the different radiation beams in the order γ > C > Fe, thus exhibiting an inverse correlation with LET [γ(0.2 keV/μm) < C (13keV/μm) < Fe (200 keV/(μm)]. Consistent with in vitro data, the yield of chromosomal DNA DSBs decreased with increasing LET in Chinese hamster cells irradiated with carbon ion beams with different LETs, suggesting that the decrease in the yield of clustered damage with increasing LET is not peculiar to in vitro irradiation of DNA, but is common for both in vitro and in vivo irradiation. These results suggest that the adverse biological effect of the ionizing radiation is not simply accounted for by the yield of clustered DNA damage, and that the complexity of the clustered damage needs to be considered to understand the biological consequences of ionizing radiation.

• 日本放射線影響学会の論文
• 2008-03-16

著者

• FURUSAWA Yoshiya

  Heavy-ion Radiobiology Research Group, Research Center for Charged Particle Therapy, National Instit

• Futami Yasuyuki

  茨城県立医療大学 放射線技術科学科

• Futami Yasuyuki

  The Institute Of Physics University Of Tsukuba

• HIRAYAMA Ryoichi

  Heavy-Ion Radiobiology Research Group, Research Center for Charged Particle Therapy, National Instit

• Iwai Shigenori

  Division Of Chemistry Graduate School Of Engineering Science Osaka University

• Ide Hiroshi

  Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University

• TERATO Hiroaki

  Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University

• TANAKA Ruri

  Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University

• NAKAARAI Yusuke

  Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University

• NOHARA Tomonori

  Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University

• DOI Yusuke

  Divisionof Chemistry, Graduate School of Engineering Science, Osaka University

• Hirayama Ryoichi

  Heavy-ion Radiobiology Research Group Research Center For Charged Particle Therapy National Institut

• Hirayama Ryoichi

  Heavy-ion Radiobiol. Res. Gr. Natl. Inst. Radiol. Sci.

• Doi Yusuke

  Divisionof Chemistry Graduate School Of Engineering Science Osaka University

• Furusawa Yoshiya

  高エネルギー加速器研究機構物質構造科学研究所

• Terato Hiroaki

  Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University

• Tanaka Ruri

  Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University

• Furusawa Yoshiya

  Heavy-ion Radiobiology Res. Group National Inst. Of Radiological Sciences

• Nakaarai Yusuke

  Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University

• Ide Hiroshi

  Department Of Chemistry Faculty Of Science Kyushu University

• Nohara Tomonori

  Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University

• Furusawa Yoshiya

  Div. Of Accelerator Res. Natl. Inst. Of Radiol. Sci.

• Furusawa Yoshiya

  Heavy-ion Radiobiol. Res. Gr. Res. Center Charged Particle Therapy Nirs.

• Doi Yusuke

  Division Of Environmental Science And Technology Graduate School Of Agriculture Kyoto University

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