Relationship between Aberration Yield and Mitotic Delay in Human Lymphocytes Exposed to 200 MeV/u Fe-ions or X-rays
スポンサーリンク
概要
- 論文の詳細を見る
The time-course of Fe-ion (200 MeV/u, 440 keV/μm) and X-ray induced chromosomal damage was investigated in human lymphocytes. After cells were exposed in G_0 and stimulated to grow, aberrations were measured in first-cycle metaphases harvested 48, 60 and 72h post-irradiation. Additionally, lesions were analysed in G_2 and mitotic (M) cells collected at 48h using calyculin A-induced premature chromosome condensation (PCC). Following X-irradiation, similar aberration yields were found in all of the samples scored. In contrast, after Fe-ion exposure a drastic increase in the aberration frequency with sampling time was observed, i.e. cells arriving late at the first mitosis carried more aberrations than those arriving at earlier times. The PCC data indicate that the delayed entry of heavily damaged cells into mitosis observed after Fe-ion irradiation resulted from a prolonged arrest in G_2. Altogether these experiments provide further evidence that in the case of high-LET exposure cell-cycle delays of severely damaged cells have to be taken into account for any meaningful quantification of chromosomal damage and, consequently, for an accurate estimate of the RBE.
- 日本放射線影響学会の論文
著者
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FURUSAWA Yoshiya
Heavy-ion Radiobiology Research Group, Research Center for Charged Particle Therapy, National Instit
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安藤 興一
学振科技特
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安藤 興一
Natl. Instit. Rad. Sci. Med.
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FURUSAWA Yoshiya
National Institute of Radiological Sciences
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Futami Yasuyuki
Dvisin Of Accelerator Physics And Engineering National Institite Of Radiological Sciences Chiba Japa
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Ando Koichi
Heavy-ion Radiobiology Research Group National Institute Of Radiological Sciences
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Ritter Sylvia
Dep. Of Biophysics Gesellschaft Fuer Schwerionenforschung (gsi)
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Ando K
Division Of Health Sciences Graduate School Of Medicine Osaka University
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Ando Koichi
Heavy-ion Radiobiology Res. Group National Inst. Of Radiological Sciences
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Ando Koichi
Department Of Technical Support And Development Natl. Inst. Radiol. Sci.
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Ando Koichi
Space And Particle Radiation Science Research Group National Institute Of Radiological Sciences
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Ritter Sylvia
Gesellschaft Fur Schwerionenforschung (gsi)
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RITTER SYLVIA
Biophysik
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NASONOVA ELENA
Biophysik
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Ando Koichi
Division Of Health Sciences Graduate School Of Medicine Osaka University
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Furusawa Y
Research Center For Charged Particle Therapy National Institute Of Radiological Sciences
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Nasonova Elena
Biophysik:department Of Radiation And Radiobiological Research Jinr
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Furusawa Yoshiya
Heavy-ion Radiobiology Res. Group National Inst. Of Radiological Sciences
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Ando Koichi
Heavy-ion Radio. Res. Nirs
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Furusawa Yoshiya
Heavy-ion Radiobiol. Res. Gr. Res. Center Charged Particle Therapy Nirs.
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