Chromosome Breakage and Cell Lethality in Human Hepatoma Cells Irradiated with X rays and Carbon-ion Beams

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概要

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• Prediction of radiosensitivity would be valuable for heavy-ion radiotherapy. Premature chromosome condensation (PCC) technique has been a potential predictive assay in photon radiotherapy, but has not been investigated for hepatomas receiving heavy ions. Two human hepatoma cell lines, i.e., HLE and HLF, were irradiated with either 290 MeV/u carbon ions or 200 kVp X rays. Cell lethality was assayed by colony formation and compared with the unrejoined fraction of chromatin breaks as measured by PCC technique. Carbon ions at linear energy transfer (LET) of 76 keV/μm produced cell death more effectively than those of 13 keV/μm and X rays. For the cell killing, the relative biological effectiveness (RBE) of 13 and 76 keV/μm carbon ions compared with X rays was 1.10-1.24 and 2.57-2.59, respectively. Mean number of chromosomes in HLE and HLF cells was similar to each other, i.e., 60.48 and 60.28. RBEs for chromatin breaks of 13 and 76 keV/μm carbon ions were 1.30-1.31 and 2.64-2.79, respectively. A strong correlation between unrejoined chromatin breaks and cell killing for human hepatoma cells was observed irrespective of radiation quality. We conclude that PCC provides a potential predictor for the radiosensitivity of individual hepatoma that are treated with photon as well as heavy ion irradiation.

著者

• Isono Kaichi

  Second Department Of Surgery Chiba University School Of Medicine

• Isono Kaichi

  船橋市立医療センター

• Isono Kaichi

  Second Department Of Surgery School Of Medicene Child University

• Suzuki Minoru

  Particle Radiation Oncology Research Center Kyoto University

• 安藤 興一

  学振科技特

• Suzuki M

  Particle Radiation Oncology Research Center Kyoto University

• OCHIAI Takenori

  Second Department of Surgery, Chiba University School of Medicine

• Ochiai Takenori

  Second Department Of Surgery Chiba University School Of Medicine

• Kase Youko

  National Institute Of Radiological Sciences

• OFUCHI TORU

  Space and Particle Radiation Science Research Group, National Institute of Radiological Sciences

• SUZUKI MASAO

  Space and Particle Radiation Science Research Group, National Institute of Radiological Sciences

• KASE YOKO

  Space and Particle Radiation Science Research Group, National Institute of Radiological Sciences

• ANDO KOICHI

  Space and Particle Radiation Science Research Group, National Institute of Radiological Sciences

• Ando Koichi

  Department Of Technical Support And Development Natl. Inst. Radiol. Sci.

• Ando Koichi

  Space And Particle Radiation Science Research Group National Institute Of Radiological Sciences

• Suzuki Masao

  Heavy-ion Radiobiology Res. Group National Inst. Radiological Sciences

• Kase Yoko

  Space And Particle Radiation Science Research Group National Institute Of Radiological Sciences

• Ando Koichi

  Division Of Health Sciences Graduate School Of Medicine Osaka University

• Ofuchi Toru

  Space And Particle Radiation Science Research Group National Institute Of Radiological Sciences:seco

• Kase Yoko

  Research Center Of Charged Particle Therapy Natl Inst Radiol Sci.

• Ochiai Takenori

  Second Department Of Surgery School Of Medicine Chiba University

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