Mutation Induction in Cultured Human Cells after Low-dose and Low-dose-rate γ-ray Irradiation : Detection by LOH Analysis

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概要

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• To study the genetic effects of low-doses and low-dose-rate ionizing radiation (IR), human lymphoblastoid TK6 cells were exposed to 30 mGy of γ-rays at a dose-rate of 1.2 mGy/hr. The frequency of early mutations (EMs) in the thymidine kinase (TK) gene locus was determined to be 1.7×10^<-6>, or 1.9-fold higher than the level seen in unirradated controls. These mutations were analyzed with a loss of heterozygosity (LOH) detection system, a methodology which has been shown to be sensitive to the effects of radiation. Among the 15 EMs observed after IR exposure, 8 were small interstitial-deletion events restricted to the TK gene locus. However, this specific type of event was not found in unirradiated controls. Although these results were observed under the limited conditions, they strongly suggest that the LOH detection system can be used for estimating the genetic effects of a low-dose IR exposure delivered at a low-dose-rate.

• 日本放射線影響学会の論文
• 2007-01-15

著者

• YATAGAI Fumio

  RIKEN Institute

• HONMA Masamitsu

  Natl. Inst. Health Sci.

• SUZUKI Hiromi

  Japan Space Forum

• SHIMAZU Toru

  Japan Space Forum

• Suzuki Minoru

  Particle Radiation Oncology Research Center Kyoto University

• Suzuki M

  Particle Radiation Oncology Research Center Kyoto University

• ISHIOKA Noriaki

  Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)

• Ishioka N

  Iss Science Project Office Institute Of Space And Astronautical Science Japan Aerospace Exploration

• Ishioka Noriaki

  奈良県立医科大学 生物学

• Ishioka Noriaki

  National Space Development Agency Of Japan

• Ishioka Noriaki

  京都工芸繊維大学 工芸科学部応用生物学

• HONMA Masamitsu

  Division of Genetics and Mutagenesis, National Institute of Health Sciences

• Seki Masaya

  Heavy-ion Radiobiology Research Group Research Center For Charged Particle Therapy National Institut

• Iwaki Masaya

  Advanced Development And Support Center The Institute Of Physical And Chemical Research

• Ito Nobuhiko

  Laboratory Of Veterinary Radiology And Radiation Biology Department Of Veterinary Medicine Kitasato

• UMEBAYASHI Yukihiro

  Advanced Development and Support Center, The Institute of Physical and Chemical Research

• SUZUKI Masao

  International Space Radiation Laboratory, National Institute of Radiological Sciences

• SUZUKI Hiromi

  Space Utilization Promotion Department, Japan Space Forum

• SHIMAZU Toru

  Space Utilization Promotion Department, Japan Space Forum

• YATAGAI Fumio

  Advanced Development and Support Center, The Institute of Physical and Chemical Research

• Ishioka Noriaki

  Institute Of Space And Astronautical Science Japan Aerospace Exploration Agency (jaxa)

• Yatagai Fumio

  Inst. Phys. Chem. Res.

• Yatagai Fumio

  Advanced Development And Support Center Riken Institute

• Yatagai Fumio

  Division Of Radioisotope Technology The Institute Of Physical And Chemical Research

• Yatagai Fumio

  Ri Inst. Phys. Chem. Res.

• Shimazu Toru

  Life Science Space Utilization & Res. Dept. Japan Space Forum

• Suzuki Masao

  National Inst. Radiological Sci. Chiba Jpn

• Ishioka Noriaki

  Space Utilization Research Center National Space Development Agency Of Japan (nasda)

• Ishioka Noriaki

  Department Of Space Environmental Medicine Kagoshima University Graduate School Of Medical And Denta

• Suzuki Masao

  Heavy-ion Radiobiology Research Group Research Center For Charged Particle Therapy National Institut

• Yatagai Fumio

  理化学研究所

• Honma M

  Division Of Genetics And Mutagenesis National Institute Of Health Sciences

• Umebayashi Yukihiro

  Advanced Development And Support Center The Institute Of Physical And Chemical Research

• Yatagai F

  Riken Institute

• Honma Masamitsu

  Division Of Genetics And Mutagenesis National Institute Of Health Science

• Suzuki Masao

  International Space Radiation Laboratory National Institute Of Radiological Science

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