Expression of p53-Regulated Proteins in Human Cultured Lymphoblastoid TSCE5 and WTK1 Cell Lines during Spaceflight
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概要
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The aim of this study was to determine the biological effects of space radiations, microgravity, and the interaction of them on the expression of p53-regulated proteins. Space experiments were performed with two human cultured lymphoblastoid cell lines: one line (TSCE5) bears a wild-type p53 gene status, and another line (WTK1) bears a mutated p53 gene status. Under 1 gravity or microgravity conditions, the cells were grown in the cell biology experimental facility (CBEF) of the International Space Station for 8 days without experiencing the stress during launching and landing because the cells were frozen during these periods. Ground control samples were simultaneously cultured for 8 days in the CBEF on the ground for 8 days. After spaceflight, protein expression was analyzed using a PanoramaTM Ab MicroArray protein chips. It was found that p53-dependent up-regulated proteins in response to space radiations and space environment were MeCP2 (methyl CpG binding protein 2), and Notch1 (Notch homolog 1), respectively. On the other hand, p53-dependent down-regulated proteins were TGF-β, TWEAKR (tumor necrosis factor-like weak inducer of apoptosis receptor), phosho-Pyk2 (Proline-rich tyrosine kinase 2), and 14-3-3θ/τ which were affected by microgravity, and DR4 (death receptor 4), PRMT1 (protein arginine methyltransferase 1) and ROCK-2 (Rho-associated, coiled-coil containing protein kinase 2) in response to space radiations. ROCK-2 was also suppressed in response to the space environment. The data provides the p53-dependent regulated proteins by exposure to space radiations and/or microgravity during spaceflight. Our expression data revealed proteins that might help to advance the basic space radiation biology.
著者
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SUZUKI Hiromi
Japan Space Forum
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SHIMAZU Toru
Japan Space Forum
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SEKI Masaya
Space Environmental Medicine, Kagoshima University, Graduate School of Medical and Dental Sciences
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OHNISHI Takeo
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA)
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Ono Koji
Particle Radiation Oncology Research Center Kyoto University
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Omori Katsunori
Institute Of Space And Astronautical Science Japan Aerospace Exploration Agency (jaxa)
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Ishioka Noriaki
Institute Of Space And Astronautical Science Japan Aerospace Exploration Agency (jaxa)
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Hashizume Toko
Space Environmental Medicine Kagoshima University Graduate School Of Medical And Dental Sciences
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TAKAHASHI Akihisa
Advanced Scientific Research Leaders Development Unit, Gunma University
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Takahashi Akihisa
Advanced Scientific Research Leaders Development Unit Gunma University
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OMORI Katsunori
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency
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SEKI Masaya
Space Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University
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HASHIZUME Toko
Space Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University
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ISHIOKA Noriaki
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency
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OHNISHI Takeo
Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency
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