Effect of Space Flight on the Frequency of Micronuclei and Expression of Stress-responsive Proteins in Cultured Mammalian Cells
スポンサーリンク
概要
- 論文の詳細を見る
Results of past space experiments suggest that the biological effect of space radiation could be enhanced under microgravity in some cases, especially in insects. To examine if such a synergistic effect of radiation and microgravity also exists in human cells, frequencies of chromosome instability and cellular levels of several stress-responsive proteins were analyzed in cultured human and rodent cells after space flight. Human (MCF7 and AT2KY), mouse (m5S) and hamster (SHE) cell lines were loaded on the Space Shuttle Discovery (STS-95 mission) and grown during a 9-day mission. After landing, the micronuclei resulting from abnormal nuclear division and accumulation of stress-responsive proteins such as p53 and mitogen-activated protein kinases (MAPKs), which are involved in radiation-induced signal transduction cascades, were analyzed. The frequencies of micronuclei in all the four mammalian cell strains tested were not significantly different between flight and ground control samples. Also, the cellular amounts of p53, p21 (WAF1/SDI1/CIP1) and activated (phosphorylated) forms of three distinct MAPKs in MCF7 and m5S cells of flight samples were similar to those of ground control samples. These results indicated that any effect of space radiation, microgravity, or combination of both were not detectable, at least under the present experimental conditions.
- 日本放射線影響学会の論文
著者
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SHIMAZU Toru
Japan Space Forum
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Suzuki Fumio
Fukujuji Hospital
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Suzuki Fumio
関西外国語大学
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Ishioka Noriaki
奈良県立医科大学 生物学
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Ishioka Noriaki
京都工芸繊維大学 工芸科学部応用生物学
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Suzuki Fumio
First Department of Internal Medicine
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ISHIZAKI Kanji
Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute
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西谷 和彦
東北大学大学院生命科学研究科
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西谷 和彦
東北大 大学院
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石崎 寛治
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute
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Ishizaki Kanji
Central Laboratory And Radiation Biology Research Institute Aich Cancer Center
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Ishizaki Kanji
Aichi Cancer Center Research Institute
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Ishizaki K
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute
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Ishizaki Kanji
Laboratory Of Experimental Radiology Aichi Cancer Center
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Ishizaki Kanji
Aichi Cancer Center
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KATO Tomohisa
Radiat Biol Center, Kyoto Univ
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Kato Takesi
School Of Medicine Osaka University
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Kato Tomohisa
関西外国語大学
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Kato Tomohisa
Radiation Biology Center Kyoto University
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Kato Tomohisa
Radiat Biol Center Kyoto Univ
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FUKUI Keiji
Japan Space Forum
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KAMIGAICHI Shigeki
Space Utilization Research Center, National Space Development Agency of Japan
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ISHIOKA Noriaki
Space Utilization Research Center, National Space Development Agency
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Han Zhen-bo
関西外国語大学
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Han Zhen-bo
Radiation Biology Center Kyoto University
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IKENAGA MITUO
Radiation Biology Center, Kyoto University
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HIRAYAMA JUN
Radiation Biology Center, Kyoto University
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KITAO HIROYUKI
Radiation Biology Center, Kyoto University
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NISHIZAWA KIMIKO
Aichi Cancer Center Research Institute
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SUZUKI FUMIO
Research Institute for Radiation Biology and Medicine, Hiroshima University
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CANNON THOMAS
Department of Space Biosciences, Walter Reed Army Institute of Research
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MATSUMIYA HIROYUKI
Space Utilization Research Center, National Space Development Agency
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Kitao Hiroyuki
Radiation Biology Center Kyoto University
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Kamisaka S
Graduate School Of Science And Engineering University Of Toyama
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Ishizaki K
Aichi Cancer Center Research Institute
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Kato T
Radiation Biology Center Kyoto University
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Kato Tomohisa
Radiat. Biol. Center Kyoto University
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Ishioka Noriaki
Space Utilization Research Center National Space Development Agency Of Japan (nasda)
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Ishioka Noriaki
Department Of Space Environmental Medicine Kagoshima University Graduate School Of Medical And Denta
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Ishizaki Kanji
大阪府立大学先端科学イノベーションセンター 放射線生命科学
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Ikenaga M
Radiation Biology Center Kyoto University
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Ikenaga Mituo
関西外国語大学
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Suzuki Fumio
Mitsubishi Electric Corporation
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Suzuki Fumio
Research Institute For Radiation Biology And Medicine Hiroshima University
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Suzuki Fumio
Department Of Regulatory Radiobiology Research Institute For Radiation Biology And Medicine Hiroshim
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Nishitani Kazuhiko
Graduate School Of Life Sciences Tohoku University
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Nishitani Kazuhiko
Grad. Sch. Life Sci. Tohoku Univ.
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Fukui Keiji
Life Science Research Group Department Of Science And Applications Japan Space Forum
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Hirayama Jun
Radiation Biology Center Kyoto University
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Hirayama Jun
Radiat. Biol. Center Kyoto Univ.
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Kamigaichi S
Nasda
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Ikenaga Mituo
Radiat. Biol. Cent. Kyoto Univ.
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Cannon Thomas
Department Of Space Biosciences Walter Reed Army Institute Of Research
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Kumasaki Saori
Department Of Biology And Geosciences Graduate School Of Science Osaka City University
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Ikenaga Mitsuo
Radiation Biology Center Kyoto University
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Kamisaka Seiichiro
Department Of Biology Graduate School Of Science And Engineering University Of Toyama
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Kamigaichi Shigeki
Space Utilazation Research Center Nasda
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Matsumiya Hiroyuki
Space Utilization Research Center National Space Development Agency
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Suzuki Fumio
Division Of Cardiology Kawaguchi Kohgyo General Hospital
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Soga Kouichi
Osaka City Univ. Osaka Jpn
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Suzuki Fumio
Tokyo Med. And Dental Univ.
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Nishizawa Kimiko
Lab. Exp. Radiol. Aichi Cancer Center
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Han Zhenbo
Radiat. Biol. Center Kyoto Univ.
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