An Approach to Estimate Radioadaptation from DSB Repair Efficiency

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概要

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• In this review, we would like to introduce a unique approach for the estimation of radioadaptation. Recently, we proposed a new methodology for evaluating the repair efficiency of DNA double-strand breaks (DSB) using a model system. The model system can trace the fate of a single DSB, which is introduced within intron 4 of the TK gene on chromosome 17 in human lymphoblastoid TK6 cells by the expression of restriction enzyme I-SceI. This methodology was first applied to examine whether repair of the DSB (at the I-SceI site) can be influenced by low-dose, low-dose rate gamma-ray irradiation. We found that such low-dose IR exposure could enhance the activity of DSB repair through homologous recombination (HR). HR activity was also enhanced due to the pre-IR irradiation under the established conditions for radioadaptation (50 mGy X-ray-6 h-I-SceI treatment). Therefore, radioadaptation might account for the reduced frequency of homozygous loss of heterozygosity (LOH) events observed in our previous experiment (50 mGy X-ray-6 h-2 Gy X-ray). We suggest that the present evaluation of DSB repair using this I-SceI system, may contribute to our overall understanding of radioadaptation.Translated and modified from Radiat. Biol. Res. Comm. Vol.43(4); 443-453, (2008, in Japanese)

• 2009-09-16

著者

• YATAGAI Fumio

  RIKEN Institute

• HONMA Masamitsu

  Natl. Inst. Health Sci.

• ENOMOTO Shuichi

  RIKEN Institute

• HONMA Masamitsu

  Division of Genetics and Mutagenesis, National Institute of Health Sciences

• Yatagai Fumio

  Inst. Phys. Chem. Res.

• Yatagai Fumio

  Division Of Radioisotope Technology The Institute Of Physical And Chemical Research

• Yatagai Fumio

  Ri Inst. Phys. Chem. Res.

• YATAGAI Fumio

  Metallomics Imaging Research Unit, Center for Molecular Imaging Science, The Institute of Physical a

• SUGASAWA Kaoru

  Biosignal Research Center, Kobe University

• ENOMOTO Shuichi

  Metallomics Imaging Research Unit, Center for Molecular Imaging Science, The Institute of Physical a

• Yatagai Fumio

  理化学研究所

• Honma M

  Division Of Genetics And Mutagenesis National Institute Of Health Sciences

• Sugasawa Kaoru

  Biosignal Research Center Kobe University

• Yatagai F

  Riken Institute

• Honma Masamitsu

  Division Of Genetics And Mutagenesis National Institute Of Health Science

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