Radiation-Induced Chromosome Damage in G_1 Phase Cells as Breaks in Permature Chromosome Condensation (PCC) and Its Biological Meaning

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概要

• 論文の詳細を見る

• We studied radiation-induced chromosome fragmentation in the G_1-phase of Syrian golden hamster embryo (SHE) cells by premature chromosome condensation (PCC). SHE cells were irradiated with ^<14>N ions (95 MeV) and ^4He ions (22 MeV) generated by the cyclotron and ^<137>Cs gamma rays. The number of chromosome breaks in PCC (PCC break) induced by 14N ions was higher than in those by the others. High linear energy transfer (LET) radiation (heavy ions) is more effective in the induction of PCC breaks than low LET radiation (gamma-rays). 530 key/μm ^<14>N induced PCC 2.4 times more than ^<137>Cs gamma rays. There is an inverse relationship between the number of chromatid delations and the incidence of mutation or transformation. There is, however, a good correlation between the incidence of PCC breaks and frequen-cies of mutation and/or morphological transformation. Although over 95% of the PCC breaks induced by gamma-rays were rejoined within 8 hours of post-irradiation incubation, only 35 to 45% of PCC breaks induced by heavy ions were rejoined. These results suggest that there is a qualitative difference in the initial chromosome damage caused by heavy ions and gamma rays.

• 日本放射線影響学会の論文

著者

• Suzuki Keiji

  Division Of Radiation Biology Department Of Radiology And Radiation Biology Graduate School Of Biome

• Suzuki M

  Particle Radiation Oncology Research Center Kyoto University

• NAGATA Kenji

  Particle Radiation Oncology Research Centre, Research Reactor Institute, Kyoto University

• Watanabe M

  Laboratory Of Radiation Biology Research Reactor Institute Kyoto University

• Yatagai Fumio

  Division Of Radioisotope Technology Riken

• Yatagai Fumio

  Ri Inst. Phys. Chem. Res.

• NAKANO KAZUSHIRO

  The Institute of Physical and Chemical Research

• WATANABE Masami

  Division of Radiation Biology, Department of Radiology and Radiation Biology, Course of Life Science

• Yatagai F

  Division Of Radioisotope Technology Riken

• Watanabe M

  Nagasaki University

• SUZUKI MASAO

  Division of Radiation Biology, School of Medicine, Yokohama City University

• NAKANO KAZUSHIRO

  Division of Radiation Biology, School of Medicine, Yokohama City University

• Suzuki K

  Course Of Life Sciences And Radiation Research Graduate School Of Biomedical Sciences Nagasaki Unive

• Suzuki K

  Division Of Radiation Biology Department Of Radiology And Radiation Biology Course Of Life Sciences

• Yatagai F

  Riken Institute

• Suzuki Keiji

  Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Watanabe Masami

  Division Of Radiation Biology School Of Medicine Yokohama City University

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